实验性隐睾诱导小鼠生精细胞凋亡的研究  

THE INJURIOUS EFFECTS OF CADMIUM AND PROTECTION OF ZINC ON ULTRASTRUCTURE AND CYTOCHEMISTRY OF G 6 Pase OF VAS DEFERENS IN RATS

作　　者：徐健[1] 许增禄[1] 姜英涛 钱晓菁[1] 黄玉苓[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所

出　　处：《解剖学报》1999年第1期82-85,I018,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金;卫生部科研基金

摘　　要：目的研究手术隐睾所致成年小鼠生殖细胞凋亡及相关调节因子ｂｃｌ－２、ｂａｘ蛋白在曲细精管中的定位、变化。方法以末端脱氧核糖核酸转移酶介导的ｄＵＴＰ缺口末端标记法（ｔｅｒｍｉｎａｌｄｅｏｘｙｎｕｃｌｅｏｔｉｄｙｌｔｒａｎｓ－ｆｅｒａｓｅ－ｍｅｄｉａｔｅｄｄＵＴＰｎｉｃｋ－ｅｎｄｌａｂｅｌｉｎｇｍｅｔｈｏｄ，ＴＵＮＥＬ）检测凋亡的生殖细胞，生物素－抗生物素蛋白ＤＣＳ（ｂｉ－ｏｔｉｎ－ａｖｉｄｉｎＤｃｅｌｓｏｒｔｅｒ）体系间接免疫荧光法检测Ｂｃｌ－２、Ｂａｘ蛋白在曲细精管中的定位、分布。结果隐睾术后３ｄ，手术侧睾丸重量及细胞凋亡数量与对侧无明显区别。而６～１５ｄ，睾丸重量明显减轻，凋亡细胞明显增多，以精母细胞、精子细胞为主。在正常小鼠曲细精管中，ｂｃｌ－２大量表达，呈散在的颗粒状存在于各级生精细胞中，以精子细胞胞质为主。Ｂａｘ蛋白表达量较少，一部分也以散在的颗粒存在于各级生精细胞质中，另一部分则较致密地分布于曲细精管基底部。隐睾术后３ｄ，Ｂｃｌ－２、Ｂａｘ的表达与对侧无明显区别；６～１５ｄ，Ｂｃｌ－２明显下降，Ｂａｘ明显增加。结论隐睾明显诱导生殖细胞凋亡，Ｂｃｌ－２、Ｂａｘ蛋白对生殖细胞的凋亡有重要调节作用，提示温?Objective\ To study the injurious effects of small dose CdCl 2 and protection of zinc on proximal segment and distal segment of vas deferens in rats. Methods The rats were treated with CdCl 2(2mg/kg body weight) and Cd+Zn. The specimens were investigated by transmission electron microscopy and quantitative analysis of the changes of the cytochemical reactive product of G 6 Pase from 4 hours to 60 days after treatment. Results The ultrastructural changes of principal cells were detected after 4 hours of Cd treatment. The degeneration became most prominent within 3 7 days, but tended towards recovery after 15 days and nearly normal appearance on 60 days after Cd treatment. The main alterations in principal cells were dilatation of endoplasmic reticulum (ER), swelling of mitochondria and increasing of myelin figures. G 6 Pase reactive product of principal cells of distal segment decreased 4 hours after Cd treatment, became most prominent after 3 days, tended to increase after 15 days. The protective effects of zinc on Cd induced injuries in ultrastructure of principal cells and G 6 Pase reactive product of distal segment principal cells from vas deferens were prominent in rats. Conclusion Cadmium had injurious effects on the ultrastructure and G 6 Pase activity in principal cells of vas deferens. Zinc had prominent protective effects to the injuries of cadmium.

关 键 词：生精细胞 细胞凋亡 隐睾 小鼠 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] R697.22[医药卫生—基础医学]