细胞周期素D1基因G870A多态性与儿童急性淋巴细胞白血病的研究  

The association study between the CCND1 G870A polymorphism and risk of children with acute lymphoblastic leukemia

作　　者：戎留成[1] 徐红艳[1] 芮耀耀[1] 何璐璐[1] 陆勤[1] 李捷[2] 张正东[3] 方拥军[1] 

机构地区：[1]南京医科大学附属南京儿童医院血液肿瘤科,江苏南京210008 [2]苏州大学附属儿童医院血液肿瘤科,江苏苏州21500 [3]南京医科大学公共卫生学院分子遗传毒理实验室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2010年第12期1700-1702,1814,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市卫生局科技发展基金资助(ZKM200509359)

摘　　要：目的:研究细胞周期素Dl(cyclin D1 gene,CCNDl)G870A单核苷酸多态性与中国南方地区儿童急性淋巴细胞白血病患病危险性的关系。方法:应用聚合酶链反应一限制性片段长度多态性对346例中国南方地区白血病儿童患者和520例健康对照进行基因分型。结果:在病例和对照中CCNDl G870A分布频率无差异。在对白血病免疫分型及危险度分层分析中,该基因分布频率亦无明显的差异。结论:CCNDl G870A基因多态性与中国南方儿童急性淋巴细胞白血病患病风险无明显的相关性。Objective： To investigate the susceptibility of the cyclin D1 gene（CCNDl）G870A polymorphism to childhood acute lymphoblastic leukemia（ALL） in a southern Chinese population.Methods：Three hundred and forty-six children with ALL and 520 matched cancer-free controls were genotyped for the CCNDl G870A polymorphism by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）and sequencing.Results：The distribution of three CCND1 G870A genotypes among cases was not significantly different from that among controls.Among ALL patients,the phenotype distribution was not signifcantly different when stratified by treatment branch and immunophenotype of ALL.Conclusion：The results showed that the G870A polymorphism of CCND1 was not associated with the risk of ALL in a southern Chinese population.

关 键 词：儿童急性淋巴细胞白血病 CCND1 基因多态性 

分 类 号：R725.5[医药卫生—儿科]