外加极低频交变磁场照射下致吸附纳米FeOx颗粒不同类型肝癌细胞凋亡研究  

Apoptosis analysis of different kinds of hepatom cell lines absorbing magnetic nano FeOx exposed to extremely low frequency altering electric magnetic filed

作　　者：居会祥[1] 戴真煜[2] 孙明忠[1] 

机构地区：[1]东南大学医学院附属盐城医院医学检验科,江苏盐城224001 [2]东南大学医学院附属盐城医院医学介入放射科,江苏盐城224001

出　　处：《南京医科大学学报（自然科学版）》2010年第12期1708-1712,1819,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省盐城市科技发展计划项目(YK2008148);江苏省卫生厅科研项目(Z200915)

摘　　要：目的:观察纳米FeOx粒子在100Hz外加极低频交变磁场(extremely low frequency altering electric-magnetic field,EMF)作用下导致的Bel-7402及HepG2两种肝癌细胞凋亡现象,并探讨其分子机制。方法:采用细胞计数试剂盒(CCK-8)检测纳米粒子在EMF作用下对不同类型体外培养肝癌细胞Bel-7402及HepG2增殖的影响,流式细胞仪检测不同实验条件下细胞发生的早期凋亡及死亡,运用Western-blot检测细胞凋亡相关蛋白Bcl家族及热休克蛋白-27(Hsp-27)的表达以确定其对细胞影响的分子机制。结果:EMF照射纳米FeOx颗粒吸附HepG2和Bel-7402细胞增殖抑制率分别为(35.54±3.2)%、(76.31±2.3)%(P<0.05),凋亡率分别为(18.64±6.68)%、(23.34±2.16)%(P>0.05),两种细胞凋亡率差异不显著。Western-blot实验结果表明两种细胞的Bcl蛋白家族大量表达,细胞发生明显早期凋亡,其中Bel-7402细胞的Bcl/Bax值明显低于HepG2细胞的比值,而Hsp-27蛋白表达明显高于HepG2细胞中的表达,Bel-7402细胞的自我保护功能表现得更为显著,两种细胞的凋亡率度未出现明显差异。结论:EMF照射磁性纳米FeOx颗粒吸附得两种肝肿瘤细胞均能抑制细胞增殖并导致细胞发生大量的早期凋亡,可作为一种潜在的治疗肝脏肿瘤细胞的手段。Objective： To investigate apoptosis and related molecular mechanism caused by nano FeOx powders in hepatoma cell lines HepG2 and Bel-7402 exposed to extremely low frequency altering magnetic field（EMF）.Methods： Proliferation and apoptosis of cell lines were detected by CCK-8 kit and flow cytometer using a apoptosis kit,respectirely.Western blot was utilized to detect expression of protein associated with apoptosis and heat shock protein.Results： The proliferation of cells absorbed by nano FeOx powders was inhibited due to the EMF exposure,the inhibited ratio of HepG2 and Bel-7402 cells were（35.54±3.2）% and（76.31±2.3）%（P 〈 0.05）,respectively.The exposure of EMF induced early apoptosis of cells,the apoptosis ratios of HepG2 and Bel-7402 absorbed by nano powders were（18.64±6.68）% and（23.34±2.16）%,respectirely（P 〉 0.05）.Western blot was utilized to analyze the mechanism of cells apoptosis,and the results indicated that the expression of Bcl/Bax in Bel-7402 and HepG2 cells had no difference,but the apoptosis ratio was similar in two cell lines,the expression of Hsp-27 in Bel-7402 was higher than that in HepG2 cells.Conclusion： The inhibition of proliferation and kigh early apoptosis of Bel-7402 and HepG2 loading magnetic nano FeOx powders under EMF exposure indicated that this kind of treatment could be applied as a potential method for treatment of liver tumor in future.

关 键 词：纳米FeOx HEPG2 BEL-7402 凋亡 BAX蛋白 热休克蛋白-27 

分 类 号：Q274[生物学—细胞生物学]