SLE患者外周血单核细胞LTβR的异常表达及其影响因素初探  

Abnormal expression of LTβR on peripheral blood monocytes in patient with SLE and its possible inducing factors

作　　者：刘金生[1] 印澄[1] 张戎[2] 杨晓帆[1] 王慧娟[1] 张缪佳[3] 季晓辉[1] 

机构地区：[1]南京医科大学基础医学院微生物与免疫学系,江苏南京210029 [2]南京医科大学基础医学院基础医学实验教学中心,江苏南京210029 [3]南京医科大学第一附属医院风湿科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2010年第12期1722-1728,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金资助(08NMUM005)

摘　　要：目的:观察淋巴毒素β受体(LTβR)分子在系统性红斑狼疮(SLE)外周血单核细胞上的表达情况,同时探索能够影响其表达的因素。方法:分离SLE患者及正常健康对照者的外周血单个核细胞,流式细胞术检测LTβR分子在单核细胞上的表达,免疫磁珠法分选出单核细胞,RT-PCR方法检测其mRNA的表达;同时在体外培养细胞的U937和THP1中观察脂多糖(LPS)、佛波酯(PMA)及地塞米松(DEX)对LTβR分子表达的影响。结果:SLE患者外周血单核细胞上出现LTβR分子的异常高表达,而正常人外周血单核细胞上没有表达;体外细胞培养发现,地塞米松对U937细胞上的LTβR的表达具有上调作用,而对THP1细胞和正常对照者外周血单核细胞没有上调LTβR的表达;PMA可使U937和THP1细胞膜上LTβR出现一过性的降低,但不影响其mRNA水平,对正常人单核细胞无影响;LPS对U937和THP1细胞及正常人单核细胞的LTβR表达均无影响。结论:LTβR分子在SLE患者来源的外周血单核细胞上具有异常的表达,该分子的表达可能不是仅仅由于单核细胞活化或应用激素类药物引起的;但地塞米松可能对发育早期的单核细胞的LTβR表达具有一定的上调作用。Objective： To observe expression of lymphotoxin beta receptor（LTβR） molecule on monocytes from peripheral blood of systemic lupus erythematosus（SLE） patients,and to explore regulating factors.Methods：Peripheral blood mononeuclear cells（PBMCs） from the patients and controls were separated,and then LTβR molecule expression was detected by FACS.Monocyte were separated from PBMC by the immunomagnetic beads,and then the mRNA level of LTβR was detected by RT-PCR.Besides,the changes of LTβR expression on the monocyte cell lines（U937 and THP1） and nomal peripheral blood monocyte were investigated after stimulation with LPS,PMA or DEX in vitro.Results：The PBMCs from SLE patients expressed abnormaly high levels of LTβR molecule,which did not express on the PBMCs from normal controls.In cell culture in vitro,DEX up-regulated the espression of LTβR on U937 cell,but did not on THP1 cell and normal peripheral blood monocyte.In U937 and THP1 cell cultures,LTβR expression on cell membrane displayed a temporary decrease,but the LTβR mRNA did not show any changes after treatment with PMA;while no changes were found in LTβR expression on normal peripheral blood monocyte after PMA treatment.Neither the U937 and THP1 cell lines,nor the normal peripheral blood monocyte,did not display any changes in LTβR expression after LPS treatment.Conclusion：The LTβR molecule highly expresses on peripheral blood monocyte from SLE patient,and this abnormal expression may be not related to the active of monocyte or the treatment of glucocorticoid medicine.On the other hand,DEX may be a factor that can influence this molecule expression of monocyte in an earlier stage of development.

关 键 词：系统性红斑狼疮 淋巴毒素β受体 单核细胞 

分 类 号：R593.241[医药卫生—内科学]