大鼠中枢神经元内免疫神经内分泌物质的共存  

GOEXISTENCE OF IMMUNE NEURO ENDOCRINE SUBSTANCES IN THE RAT CENTRAL NEURONS

作　　者：朱长庚[1] 刘庆莹[1] 魏瑛[1] 马春玲[1] 郝建东[1] 晏平[1] 

机构地区：[1]同济医科大学解剖学教研室

出　　处：《解剖学报》1999年第2期100-102,I001,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金

摘　　要：目的研究白细胞介素－２、代谢型谷氨酸受体１亚型（ｍＧｌｕＲ１）和雌激素受体（ＥＲ）在中枢神经系统神经细胞的表达，证实它们共存的可能性。方法使用种属特异性１抗（山羊抗ＩＬ－２、兔抗ｍＧｌｕＲ１、小鼠抗ＥＲ血清）的免疫细胞化学三重标记技术，在相邻的两张连续切片（１张显示ＩＬ－２，另１张显示ｍＧｌｕＲ１和ＥＲ）上证实大鼠大脑皮质、延髓和脊髓内上述３种物质的共存。结果在上述部位的中枢神经元内可见ＩＬ－２、ｍＧｌｕＲ１和ＥＲ３种免疫反应性标记细胞。ＩＬ＼｜２免疫反应产物为棕褐色，位于胞浆内。相邻切片的ｍＧｌｕＲ１免疫反应产物为蓝黑色，位于胞膜；ＥＲ免疫反应产物亦为棕褐色，位于胞浆或核内。显示ｍＧｌｕＲ１和ＥＲ的同一切片上有ｍＧｌｕＲ１／ＥＲ双标细胞，双标细胞约占全部标记细胞的５０％～６０％。通过对相邻的两张切片的投影核对，证实存在ｍＧｌｕＲ１／ＥＲ／ＩＬ－２三标细胞，三标细胞占ｍＧｌｕＲ１／ＥＲ双标细胞的３０％。结论在大鼠中枢神经系统内ｍＧｌｕＲ１、ＥＲ和ＩＬ－２可共存于同一个神经细胞，从而首次在细胞水平为神经免疫内分泌网络学说提供了形态学依据。Objective\ To investigate the expression of Interleukin 2(IL 2),metabotropic glutamate receptor subunit 1(mGluR1) and estrogen receptor (ER) in neurons of the rat central nervous system (CNS) and identify the coexistence possibility of these immune neuro endocrine substances in the central neurons.Methods\ The triple labeling immunocytochemical staining technique with different species specific primary antibodies(goat anti IL 2 serum,rabbit anti mGluR1 serum and mouse anti ER serum)were used to incubate two serial neighbor sections(one for demonstrating IL 2,another for mGluR1 and ER) and identify the coexistence of above three kinds of substance in the cerebral cortex,medulla oblongata and spinal cord.Results\ There were IL 2, mGluR1 |and ER immunoreactive(ir)labeled neurons in the above mentioned central areas.The IL 2 ir production showed brown in color,locating in the cytoplasm.In the neighbor serial section,mGluR1 ir production showed blue black in color,locating at the cell membrane;the ER ir production also showed brown color,locating in the cytoplasm or nucleus.There were mGluR1/ER double labeled cells in same section, which accounted for about 50% 60% of the total single and double labeled neurons.It was identified by projection check of serial neighbor sections that there were mGluR1/ER/IL 2 triple labeled cells which accounted for about 30% of total mGluR1/ER double labeled nuurons.Conclusion\ mGluR1,ER and IL 2 can coexist in same rat central neurons,therefore,providing morphological basis for the theory about immune neuro endocrine network at the cellular level for the first time.

关 键 词：免疫 神经 内皮泌 IL-2 MGLUR1 ER 神经元 

分 类 号：R392.1[医药卫生—免疫学] R338[医药卫生—基础医学]