α7nAChR激动剂-缺血后处理对大鼠心肌缺血再灌注损伤的影响  

Effects of postconditioning with a7 nicotinic acetylcholine receptor agonist and ischemia on myocardial ischemia-reperfusion injury in rats

作　　者：熊军[1] 薛富善[1] 袁玉静[1] 王强[1] 廖旭[1] 李杉[1] 王卫利[1] 刘建华[1] 

机构地区：[1]中国医学科学院北京协和医学院整形外科医院麻醉科,100144

出　　处：《中华麻醉学杂志》2010年第9期1118-1121,共4页Chinese Journal of Anesthesiology

摘　　要：目的 探讨α7烟碱型乙酰胆碱受体（α7nAChR）激动剂-缺血后处理对大鼠心肌缺血再灌注损伤的影响.方法 成年雄性SD大鼠50只,体重290～320 g,随机分为5组（n=10）：假手术组（S组）、缺血再灌注组（IR组）、缺血后处理组（IP组）、α7nAChR激动剂后处理组（P组）和α7nAChR激动剂-缺血后处理组（PIP组）.IR组结扎左冠状动脉前降支30 min、再灌注180 min制备心肌缺血再灌注损伤模型;S组仅穿线不结扎;缺血30 min时IP组再灌注10 s缺血10 s,重复3次,P组腹腔注射PNU282987 2.4 mg/kg,PIP组腹腔注射PNU282987 2.4 mg/kg后行缺血后处理.于再灌注180 min时抽取右颈内静脉血样后处死大鼠,取心脏,采用ELISA法测定血清心肌肌钙蛋白Ⅰ（cTnI）、TNF-α和高迁移率族蛋白Ⅰ（HMGB1）的浓度,采用伊文蓝和TTC双重染色法测定心肌梗死体积.结果 与S组比较,其余各组心肌梗死体积、IR组血清cTnI、TNF-α和HMGB1浓度升高（P＜0.05）;与IR组比较,IP组、P组和PIP组心肌梗死体积、血清cTnI、TNF-α和HMGB1浓度降低（P＜0.05）;与[P组比较,PIP组心肌梗死体积、P组和PIP组血清cTnI、TNF-α和HMGB1浓度降低（P＜0.05）;与P组比较,PIP组心肌梗死体积、血清TNF-α和HMGB1浓度降低（P＜0.05）.结论 α7nAChR激动剂-缺血后处理减轻大鼠心肌缺血再灌注损伤的效应较单独应用时强.Objective To investigate the effects of postconditioning with α7 nicotinic acetylcholine receptor （α7nAChR） agonist and ischemia on myocardial ischemia-reperfusion （IR） injury in rats. Methods Fifty adult male SD rats weighing 290-320 g were randomly divided into 5 groups （ n = 10 each）： Ⅰ sham operation group, Ⅱ IR group, Ⅲ ischemic postconditioning group, Ⅳ α7nAChR agonist postconditioning group and Ⅴpostconditioning with α7nAChR agonist and ischemia group. Myocardial I/R was induced by ligation of anterior descending branch of left coronary artery for 30 min followed by 1 80 min of reperfusion. In group] the anterior descending branch was only exposed but not ligated. In group Ⅲ the hearts were subjected to 3 episodes of 10 second ischemia at 10 second intervals at the end of 30 min ischemia before 180 min reperfusion, Intraperitoneal PNU282987 2.4 mg/kg was injected at the end of 30 min ischemia before 180 min reperfusion in group Ⅳ and Ⅴ .Blood samples were taken from right internal jugular vein at 180 min of reperfusion. Then the rats were killed and hearts removed to determine the concentrations of serum cardiac troponin-I （cTnI）, TNF-α and high-mobility group box 1 （HMGB1） by ELISA. The infarction size was measured by Evans blue and triphenyltetrazolium chloride staining. Results The infarction size was significantly larger in the other groups and concentrations of serum cTrI, TNF-α and HMGB1 were significantly higher in group Ⅱ than in group Ⅰ （ P 〈 0.05）. The infarction size was significantly smaller and concentrations of serum cTnI, TNF-α and HMGBI were significantly lower in group Ⅲ, Ⅴ than in group Ⅱ （P 〈 0.05）. The infarction size was significantly smaller in group Ⅴ and concentrations of serum cTnI, TNF-α and HMGB1 were significantly lower in group Ⅳ and Ⅴ than in group Ⅲ （P 〈0.05）. The infarction size was significantly smaller and concentrations of serum cTnI, TNF-α and HMGB1 were significantly lower in group Ⅴ th

关 键 词：受体 烟碱 心肌再灌注损伤 缺血后处理 

分 类 号：R541[医药卫生—心血管疾病]