胎盘TLR3分布及与HBV宫内感染的关系初探  

Relationship between TLR3 Expression in Placenta and Intrauterine Infection of Hepatitis B Virus

作　　者：王琰[1] 闫永平[2] 杜鹃[1] 苏海霞[2] 徐德忠[2] 

机构地区：[1]吉林医药学院公共卫生系流行病学教研室,132013 [2]第四军医大学预防医学系流行病学教研室

出　　处：《天津医药》2010年第12期1028-1031,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助重点项目(项目编号:30230320)

摘　　要：目的:了解Toll样受体(TLR)3在外周血HBsAg阳性母亲胎盘组织中的表达及分布,探讨其与HBV宫内感染的关系。方法:采用SABC免疫组织化学方法结合显微镜观察胎盘组织HBsAg和TLR3的表达和分布,以胎盘各层细胞出现棕黄色颗粒判断为阳性;Abbott化学发光法检测新生儿外周血HBsAg;ELISA法检测母亲外周血HBsAg和HBeAg。结果:24例母亲外周血HBsAg均阳性,除2例因胎盘组织脱落不计外,22例中17例胎盘组织中HBsAg呈阳性,6例新生儿发生HBV宫内感染,宫内感染率25%(6/24),胎盘HBV感染率77.27%(17/22)。HBsAg分布于蜕膜细胞、滋养层细胞、绒毛间质细胞、绒毛毛细血管内皮细胞。17例HBsAg阳性的胎盘组织中16例TLR3阳性,阳性细胞较少,主要在滋养层细胞和绒毛间质细胞。1例宫内感染的新生儿、HBsAg阴性的胎盘组织5例中的4例、外周血HBsAg阴性的6例正常胎盘组织切片上及空白对照均未检测到TLR3阳性表达。结论:外周血中HBV对TLR3可能仅有微弱的激活作用。Objective：To study the expression and distribution of toll-like receptor 3（TLR3） in human placenta of hepatitis B virus（HBV）intrauterine infection,and provide scientific evidence of prevention in HBV intrauterine infection.Methods：Twenty-four full-term placental tissues of HBsAg carriers and 6 of HBsAg sero-negative pregnant women were collected.The expressions of HBsAg and TLR3 were detected in placental tissues by streptavidin biotin peroxidase complex（SABC） immunohistochemical staining.The neonatal HBsAg was tested by chemiluminescence.The levels of HBsAg and HBeAg were detected in maternal serum by ELISA.Results：The serum HBsAg was positive in 24 pregnant women.HBsAg were detected in 17 of 22 placental tissues from HBsAg carrier mothers,6 of 24 neonates born from HBsAg carrier mothers developed HBV intrauterine infection.The transplacental intrauterine infection rate of HBV was about 25%（6/24）,including 6 neonates of HBV intrauterine infection.The HBsAg positive rate was 77.27%（17/22）.The positive signal of HBsAg located in the cytoplasm of decidual cells,trophoblastic cells,villous mesenchymal cells and villous capillary endothelial cells.TLR3 expressed in 16 of 17 HBsAg-positive placental tissues.The positive signal of TLR3 mainly located in the cytoplasm of trophoblastic cells and villous mesenchymal cells in the HBsAg-positive placental tissues.The expressions of TLR3 were not found in the placenta of 1 HBV intrauterine infection neonates,4 HBsAg-negative placenta,6 HBsAg sero-negative pregnant women,and blank groups.Conclusion：The results suggested that there may be only weak response of TLR3 to HBV stimulation.

关 键 词：肝炎病毒 乙型 肝炎e抗原 乙型 肝炎表面抗原 乙型 受体 细胞表面 疾病传播 垂直 母体胎儿间交换 胎盘 TOLL样受体 

分 类 号：R714.246[医药卫生—妇产科学]