胰岛素对RF/6A细胞VEGF受体表达的影响  

The Effects of Insulin on the Expression of VEGFR in RF/6A Cells

作　　者：周赛君[1] 陈松[2] 于珮 于德民[1] 

机构地区：[1]天津医科大学代谢病医院,卫生部激素与发育重点实验室,300070 [2]天津市眼科医院

出　　处：《天津医药》2010年第12期1065-1067,I0010,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30971393);天津市科技支撑重点项目(项目编号:08ZCGYSF01700);天津市卫生局科技基金重点项目及面上项目(项目编号:09KZ01,09KZ89);天津医科大学科技基金资助项目(项目编号:2007ky15,2009ky25)

摘　　要：目的:探讨胰岛素对视网膜血管内皮细胞RF/6A血管内皮细胞生长因子受体(VEGFR)的表达及血管新生的影响。方法:分别用0、1和100nmol/L的人胰岛素处理RF/6A细胞,采用MTS实验、细胞迁移实验、管腔形成实验分别检测胰岛素对RF/6A细胞增殖、迁移、管腔形成等生物学功能,RT-PCR、Western blot技术检测VEGFR的表达、磷酸化活性。结果:胰岛素可促进RF/6A的增殖、迁移、管腔形成(P<0.05或P<0.01),促进VEGFR2 mRNA的表达和VEGFR2蛋白的磷酸化(P<0.05或P<0.01),而对VEGFR1 mRNA表达的影响差异无统计学意义(P>0.05)。结论:胰岛素可经VEGFR2信号促进RF/6A细胞血管新生,而VEGFR1信号可能不参与胰岛素诱导的RF/6A细胞血管新生。Objective：To study the effect of insulin on vascular endothelial growth factor receptor（VEGFR） and angiogenesis in RF/6A retinal vascular endothelial cells,and the underlying mechanism thereof.Methods：RF/6A cells were treated with 0,1 and 100 nmol/L of insulin respectively.The MTS,cell migration and lumen formation experiments were used to detect cell proliferation,migration and lumen formation influenced by insulin in RF/6A cells respectively.RT-PCR and Western blot were used to measure the expressions of VEGFR and phosphorylation activity.Results：Insulin promoted the proliferation,migration and lumen formation in RF/6A cells（P 0.05 or P 0.01）.Insulin also increased both the expression of VEGFR2 mRNA and its phosphorylation（P 0.05 or P 0.01）.There was no significant difference in the expression of VEGFR1 mRNA between groups（P 0.05）.Conclusion：Insulin might promote angiogenesis via a VEGFR2 in RF/6A cells.VEGFR1 may not be involved in insulin-induced angiogenesis in RF/6A cells.

关 键 词：胰岛素 脉络膜 视网膜血管 内皮 血管 恒河猴 受体 血管内皮生长因子 新生血管化 病理性 

分 类 号：R977.15[医药卫生—药品]