不同方案超排卵对金黄地鼠子宫内膜组织学和超微结构的影响  

Comparison of Endometrial Histology and Ultrastructure during Different Ovarian Stimulation Protocols in Hamsters

作　　者：黄荷凤[1,2] 石一复 周馥贞[1,2] 金帆 杨树标[1,2] 何柏松 

机构地区：[1]浙江大学附属妇产科医院 [2]香港大学医学院妇产科系

出　　处：《中华妇产科杂志》1999年第6期360-362,I010,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的比较促性腺激素释放激素激动剂（ＧｎＲＨａ）辅助超排卵和单纯促性腺激素（ＰＭＳＧ）超排卵对金黄地鼠子宫内膜的影响。方法模仿人类长周期，对金黄地鼠进行ＧｎＲＨａ预处理后，用ＰＭＳＧ超排卵（１０只，ＬＡ＋ＰＭＳＧ组），并以１０只单用ＰＭＳＧ超排卵（ＰＭＳＧ组）和１０只自然周期金黄地鼠（对照组）为对照，对子宫内膜进行组织学和超微结构分析。结果组织学检查显示：ＬＡ＋ＰＭＳＧ组子宫内膜成熟度与对照组相比，差异无显著性（Ｐ＞０．０５），而ＰＭＳＧ组子宫内膜明显发育延迟（Ｐ＜０．０１）。超微结构分析显示：ＬＡ＋ＰＭＳＧ组线粒体肿大，缺少线粒体脊，无网篮样结构；ＰＭＳＧ组线粒体明显减少。结论ＧｎＲＨａ辅助超排卵方案能促进生理性子宫内膜的形成，优于单独ＰＭＳＧ超排卵方案。Objective To evaluate the influence of ovarian stimulation by pregnant mare's serum gonadotropin(PMSG) with or without gonadotropin releasing hormone agonist (GnRHa) on endometrial histology and ultrastructure in hamsters. Methods Thirty hamsters were randomly allocated into 3 groups. (1) LA+PMSG group: Leuprolide acetate (LA) was given first for desensitizing the pituitary, then PMSG added for ovarian stimulation. (2) PMSG group was injected with PMSG only. (3) Control group was given with saline of same volume as groups LA+PMSG and PMSG on the same time schedule. Endometrial histology and ultrastructure were investigated by light microscope and electron microscope. Results Comparing to the controls, the changes of endometrial histology in group of LA+PMSG was not significantly different, but its ultrastructure had marked atterations: mitochondria swelling, undifferentiated crista and no basketlike structure. Delayed endometrial maturation was frequently found in group PMSG (P<0.01), and the ultrastructure showed poorly developed mitochondria. Conclusion The degree of endometrial maturation varied considerably with different ovarian stimulation protocols. Ovarian stimulation with GnRHa was better than PMSG alone, because the protocol LA+PMSG, in hamsters, facilitated the formation of a physiologic endometrium. But the protocol with GnRHa still had a negative effect on endometrial ultrastructure.

关 键 词：子宫内膜 仓鼠 超排卵 体外受精 GNRH-A 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]