海风藤酮对小鼠抗着床作用的免疫组织化学观察  

THE INFLUENCE OF KADSURENONE UPON BLASTOCYST IMPLANTATION IN MICEIMMUNOHISTOCHEMICAL STUDIES

作　　者：吴梅[1] 王文青[1] 吴翠娇[1] 于向民[1] 潘晓亮 方丽华[1] 

机构地区：[1]青岛医学院组织学与胚胎学教研室

出　　处：《青岛医学院学报》1999年第1期4-6,F002,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省自然科学基金

摘　　要：目的观察海风藤酮对小鼠胚泡着床的影响。②方法未经产雌性昆明小白鼠于妊娠第２天，一侧子宫角内分别注射不同剂量海风藤酮（５，１０，２０，４０，６０μＬ）作为实验组，另一侧子宫角注射相应剂量的生理盐水为对照。于妊娠第８天及第１０天，取子宫组织做常规石蜡切片，ＨＥ及免疫组织化学染色，光镜观察。③结果注入海风藤酮（≥２０μＬ）组子宫蜕膜变薄，层粘连蛋白（Ｌｎ）免疫阳性物减少，纤粘连蛋白（Ｆｎ）免疫阳性物增多，并伴有不同程度胚胎发育迟缓及退化等改变。大剂量海风藤酮（≥４０μＬ）组部分动物子宫内膜无蜕膜反应，而且胚胎已消失。④结论海风藤酮通过拮抗血小板激活因子影响蜕膜反应，并使Ｌｎ及Ｆｎ的量和分布发生异常改变。这可能是该药抗着床作用的重要途径之一。\ Objective \ To investigate the influence of Kadsurenone (an antagonist of platelet activity factor PAF) upon implantation of mouse blastocyst.\ Methods \ Fiftytwo female healthy mice, mating with male healthy mice of the same breed, in proportion as 2 to 1. The 1st day of pregnancy was determined by the presence of vaginal plug. On the 2nd day of pregnancy, Kadsurenone in different dosages were injected into the uterohorn of one side of mice, equal amount of physiological saline were injected into the uterohorn of the other side. On the 8th day or the 10th day of pregnancy, the histological changes of uterus as well as fetus were observed after the tissue being treated with immunohistochemistry.\ Results \ In the experimental groups with Kadsurenone 20L, the decidua became thinner , Laminin immunoreactivity reduced, while fibronectin immunoreactivity increased, in association with retarded development and even retrogressive degeneration of mouse fetuses compared with the control groups. In groups with Kadsurenone40L, there's no decidualization of uterus, the blastocyst was digsested. \ Conclusion \ By the mechanism of antiPAF , Kadsurenone impaired the decidualization of uterus and influenced on the composition and distribution of extracellular matrix(e.g. Ln and Fn), which might be one of the mechanisms of Kadsurenone against implantation. \

关 键 词：胚泡 海风藤酮 小鼠 着床 血小板激活因子 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学] R285.5[医药卫生—基础医学]