采用gyrB基因扩增快速鉴定结核分枝杆菌复合群的初步研究  

Rapid identification of Mycobacterium tuberculosis complex by amplifying gyrB gene

作　　者：尹小毛[1] 刘志辉[1] 

机构地区：[1]广州市胸科医院肺部疾病研究所,510095

出　　处：《实用医学杂志》2010年第23期4262-4264,共3页The Journal of Practical Medicine

基　　金：国家"十一五"重大传染病专项资助项目(编号:2008ZX10003-007);广州市医药卫生科技重大项目(编号:2009-ZDa-03)

摘　　要：目的:初步分析gyrB基因扩增用于结核分枝杆菌复合群(MTC)快速鉴定的可行性。方法:采用特异性引物进行分枝杆菌标准株和临床株的gyrB基因扩增,再通过琼脂糖凝胶电泳观察聚合酶链反应(PCR)结果。结果:15株各种分枝杆菌标准株中,结核分枝杆菌(MTB)、牛分枝杆菌和非洲分枝杆菌均扩增出1184bp目的片段,而其余分枝杆菌PCR结果为阴性;94株分枝杆菌临床株中,60株MTB和10株牛分枝杆菌扩增阳性,而其他分枝杆菌(包括6株脓肿分枝杆菌、5株偶发分枝杆菌、4株龟分枝杆菌、3株鸟分枝杆菌、3株胞内分枝杆菌、2株耻垢分枝杆菌、1株堪萨斯分枝杆菌)均扩增阴性。结论:采用针对MTC的特异性引物进行gyrB基因扩增能快速、准确地鉴别MTC和非结核分枝杆菌。Objective To explore the feasibility of amplification of special sequences of gyrB gene in the identification of Mycobacterium tuberculosis complex （MTC）. Methods The gyrB genes of Mycobacterial reference strains and clinical isolates were amplified using gyrB-based species-specific primers and the results were detected by agarose gel electrophoresis. Results In 15 Mycobacterial reference strains,the target gene fragments of 1 184 bp-size were amplified successfully from Mycobacterium tuberculosis （MTB）,M. bovis,and M. africanum. Among 94 Mycoabcterial clinical isolates,amplified bands of the target gene fragments were observed on agarose gel for 60 MTB and 10 M. bovis isolates but not for 24 non-tuberculosis mycobacteria （NTM） isolates （including 6 M. abscessus strains,5 M. fortuitum strains,4 M. chelonae strains,3 M. intracellulare strains,2 M. semegmatis strains,1 M. kansasii strain）. Conclusions Amplification of gyrB gene can be used to differentiate Mycobacterium tuberculosis complex from non-tuberculosis mycobacteria rapidly and accurately.

关 键 词：分枝杆菌属 gyrB基因 结核分枝杆菌复合群 聚合酶链反应 

分 类 号：R440[医药卫生—诊断学]