荧光定量PCR检测结核杆菌DNA与涂片抗酸染色结果的比较  

A comparative analysis of the results between fluorescence quantitative PCR detection of mycobacterium tuberculosis DNA and smear acid-fast staining

作　　者：张为民[1,2] 龚文波[1,2] 

机构地区：[1]暨南大学第二临床学院 [2]广东省深圳市人民医院检验医学部,518020

出　　处：《实用医学杂志》2010年第23期4272-4274,共3页The Journal of Practical Medicine

摘　　要：目的:探讨荧光定量PCR检测结核杆菌DNA的拷贝数与涂片抗酸染色镜检阳性程度的相关性。方法:对可疑结核病患者的124份痰样本同时进行荧光定量PCR检测和涂片抗酸染色镜检,按涂片抗酸染色镜检结果阴性(-)、可疑(±)、1+、2+、3+、4+分类,对荧光定量PCR检测结核杆菌DNA的拷贝数进行统计分析。结果:98例涂片阴性痰样本中,24例样本荧光定量PCR检测阳性,结核杆菌DNA拷贝数多为102～103数量级;26例痰涂片阳性样本中,24例样本荧光定量PCR检测阳性,2例检测阴性,结核杆菌DNA拷贝数多为103～104数量级,和涂片阳性程度基本呈正相关。结论:荧光定量PCR检测结核杆菌DNA与涂片抗酸染色镜检阳性程度有较好的相关性,可以作为临床诊疗较为可靠的实验方法。Objective To explore the correlation of mycobacterium tuberculosis DNA copy number detected by fluorescence quantitative PCR（FQ-PCR） with positive degree of acid-fast staining microscopic examination. Methods 124 sputum samples from suspected TB patients were detected simultaneously by FQ-PCR for mycobacterium tuberculosis DNA and acid-fast staining microscopic examination. Copy number of mycobacterium tuberculosis DNA was analyzed according to the positive degree of acid-fast staining microscopic examination,i.e. negative（-）,suspect（±）,1＋,2＋,3＋ and 4＋. Results Among the 98 smear-negative samples,24 were positive for FQ-PCR and the DNA copy number magnitude were mostly from 102 to 103. In 26 smear-positive samples,mycobacterium tuberculosis DNA was detected from 24,the copy number magnitude were mostly from 103 to 104,and was positively correlated with the degree of acid-fast staining microscopic examination. Conclusion There is good correlation between FQ-PCR detection for mycobacterium tuberculosis DNA and positive degree of acid-fast staining microscopic examination. And FQ-PCR can be regarded as a reliable experimental method in clinical diagnosis and treatment for tuberculosis.

关 键 词：分枝杆菌 结核 荧光定量PCR 抗酸染色 DNA拷贝数 

分 类 号：R440[医药卫生—诊断学]