两步沉淀和硅胶柱层析法分离纯化卷柏穗花杉双黄酮的研究  

Two-step precipitation separation and silica gel chromatography purification of amentoflavone from Selaginella tamariscina(Beauv.)Spring

作　　者：魏丽娜[1] 袁其朋[1] 况鹏群[1] 韩雪[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《食品科技》2010年第12期172-175,共4页Food Science and Technology

摘　　要：对卷柏中穗花杉双黄酮的提取和分离纯化的研究。通过两步沉淀法初步纯化卷柏提取物粗品,采用薄层层析法选择最佳洗脱剂,用硅胶柱层析对卷柏中穗花杉双黄酮进行分离纯化研究。结果表明,采用两次沉淀法使卷柏提取物的纯度由5.92%升高至58.19%。以氯仿/甲醇(8/1,v/v)的混合溶剂为洗脱剂,得到穗花杉双黄酮产品的纯度为97.16%。Two-step precipitation separation and silica gel chromatography purification of amentoflavone from Selaginella tamariscina（Beauv.） Spring was studied.Crude extracts of Selaginella tamariscina（Beauv.） Spring were preliminarily purified by a two-step precipitation.Optimum eluent of silica gel chromatography was determined by TLC and used for separation and purification of amentoflavone from Selaginella tamariscina（Beauv.） Spring.The purity of product was increased from 5.92% to 58.19% by the two-step precipitation.Chloroform/methanol（8∶1,v/v）was used as eluent for silica gel chromatography and amentoflavone product with purity of 97.16% was obtained.

关 键 词：卷柏 穗花杉双黄酮 两步沉淀 硅胶柱层析 分离纯化 

分 类 号：R284.2[医药卫生—中药学]