miRNA-21/pG-super真核表达载体构建及其在肺泡巨噬细胞RAW264.7中的表达  

Construction of eukaryotic expression vector miRNA-21/pG-super and its expression in alveolar macrophage RAW264.7 cells

作　　者：赵锡兰[1] 汤建中[1] 杨凤琴[1] 徐文锦[1] 赵婧[1] 徐广贤[1] 

机构地区：[1]宁夏医科大学检验学院,宁夏银川750004

出　　处：《黑龙江畜牧兽医》2010年第12期20-21,24,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30960275)

摘　　要：为了研究miRNA-21在免疫细胞中的调控作用,试验构建了miRNA-21过表达载体,并将其成功转染到RAW264.7细胞中。结果表明:miRNA-21在RAW264.7细胞中获得了大量表达,说明miRNA-21/pG-super真核表达载体构建成功。To investigate regulation and control of miRNA-21 in immune cells,the overexpression vector of miRNA-21 was successfully transfected into RAW2647 cells.The results showed that miRNA-21 in RAW264.7 cells gotmuch expression;the eukaryotic expression vector miRNA-21/pG-super was successfully constructed.

关 键 词：真核表达 MIRNA-21 肺泡巨噬细胞 

分 类 号：Q812[生物学—生物工程]