大麦条纹花叶病毒中国株编码βC蛋白可以自身相互作用  

Interaction of βC protein of Barley stripe mosaic virus China strain(BSMV-CH) with itself

作　　者：孙现超[1,2] 赵文军[3] 薛杨[2] 

机构地区：[1]西南大学植物保护学院,重庆400716 [2]中国农业科学院柑桔研究所,重庆400712 [3]中国检验检疫科学研究院动植物检疫研究所,北京100029

出　　处：《植物病理学报》2010年第6期593-600,共8页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30900937);中国博士后科学基金资助项目(20090450798)

摘　　要：以分别含有BSMV-CH基因组3个组分ds-cDNA全长的pMD-α、pMD-β和pMD-γ质粒为模板,PCR扩增BSMV-CH编码各蛋白基因,克隆至酵母双杂交载体,检测各蛋白是否存在自身激活特性,用酵母双杂交分析BSMV-CH编码蛋白之间的相互作用情况,测定β-半乳糖苷酶活性确认蛋白在酵母体内的作用,Western-blot分析蛋白的体外相互作用。结果表明成功克隆到BSMV-CH编码蛋白基因,并正确克隆至酵母双杂交载体,酵母双杂交检测未发现BSMV-CH编码蛋白之间的相互作用,但确定BSMV-CH编码的βC蛋白可以自身相互作用,体外可以单体或二聚体形式存在。The protein genes of BSMV-CH were amplified by PCR from the recombined plasmids pMD-α,pMD-β and pMD-γ,which contain 3 segments of the full-length ds-cDNA of BSMV-CH respectively.The obtained fragments were cloned into vectors of yeast two-hybrid system for analyzing the protein-protein inte-ractions between BSMV-CH proteins after detecting the self-activity of each protein.The β-galacatosidase activity was tested to ensure the protein-protein interaction in the yeast cells.The protein-protein interactions in vitro were detected by western blot.The results showed that the BSMV-CH encoded protein genes were amplified and cloned into the vector of yeast two-hybrid system.No protein-protein interaction was found in yeast two-hybrid system,but the self-interaction of BSMV-CH βC protein was detected,which could keep dimer or monomer in vitro.

关 键 词：大麦条纹花叶病毒 βC蛋白 酵母双杂交 自身相互作用 

分 类 号：S432.41[农业科学—植物病理学]