应用细菌磁颗粒实时荧光RT-PCR检测南瓜花叶病毒  

Application of bacterial magnetic particles real-time RT-PCR for detecting Squash mosaic virus

作　　者：孙宁[1,2] 邓丛良[2] 么磊[2] 陈继峰[1] 

机构地区：[1]郑州大学生物工程系,郑州450001 [2]北京出入境检验检疫局,北京101312

出　　处：《植物病理学报》2010年第6期632-635,共4页Acta Phytopathologica Sinica

基　　金：国家质检总局资助(2009IK2562007IK230)

摘　　要：A novel real-time RT-PCR method,BMPs based real-time RT-PCR which integrated with magne-tic separation technique of bacterial magnetic particles(BMPs),was set up for detection of Squash mosaic virus(SqMV).After SqMV particles in crude sap were concentrated by BMPs,viral RNAs were released and detected by real time RT-PCR.The results indicated that BMPs based real-time RT-PCR was efficient,and the detection sensibility was equivalent to that of the Trizol based real-time RT-PCR,of which Trizol reagent was used for viral RNAs extration.Comparing to Trizol-based method,the BMPs-based method had advantages of simplicity on operation,time saving for RNA extraction and without using noxious organic chemicals.A novel real-time RT-PCR method,BMPs based real-time RT-PCR which integrated with magne-tic separation technique of bacterial magnetic particles（BMPs）,was set up for detection of Squash mosaic virus（SqMV）.After SqMV particles in crude sap were concentrated by BMPs,viral RNAs were released and detected by real time RT-PCR.The results indicated that BMPs based real-time RT-PCR was efficient,and the detection sensibility was equivalent to that of the Trizol based real-time RT-PCR,of which Trizol reagent was used for viral RNAs extration.Comparing to Trizol-based method,the BMPs-based method had advantages of simplicity on operation,time saving for RNA extraction and without using noxious organic chemicals.

关 键 词：南瓜花叶病毒 荧光RT-PCR检测 磁颗粒 实时 细菌 应用 MOSAIC VIRUS 

分 类 号：S432.41[农业科学—植物病理学]