花生斑驳病毒青岛分离物cp基因序列克隆与分析  

Cloning and analysis of the cp gene of two Peanut mottle virus isolates from Qing-dao

作　　者：刘媛媛[1,2] 侯珊珊[1] 常文程[1] 阴筱[1] 贾金磊[1] 李向东[1] 迟玉成[2] 

机构地区：[1]山东农业大学植物保护学院,泰安271018 [2]山东省花生研究所,青岛266100

出　　处：《植物病理学报》2010年第6期647-650,共4页Acta Phytopathologica Sinica

基　　金：新世纪优秀人才支持计划(NCET-07-0520);国家自然科学基金资助(30971895);教育部博士学科点基金(200804340006)

摘　　要：Peanut mottle virus(PeMoV) was detected via RT-PCR from two peanut samples(QD5 and QD6) with mottle symptom collected from Qingdao,Shandong Province.The 3′-terminal 892 bp fragments of their genome were cloned and sequenced.The cp genes of QD5 and QD6 were 837 bp in length and encoded 278 amino acids(aa),with DAA at aa sites regulating aphid transmission.QD5 and QD6 shared nucleotide identities of 95.3%-99.4% and aa identities of 93.5%-99.6% in cp genes with other PeMoV isolates available in the GenBank.The phylogenetic results showed that PeMoV were clustered to three groups,America,Asia and Australia,which were consistent with their geographical origins.This is the first molecular evidence on the incidence of PeMoV in China.Peanut mottle virus（PeMoV） was detected via RT-PCR from two peanut samples（QD5 and QD6） with mottle symptom collected from Qingdao,Shandong Province.The 3′-terminal 892 bp fragments of their genome were cloned and sequenced.The cp genes of QD5 and QD6 were 837 bp in length and encoded 278 amino acids（aa）,with DAA at aa sites regulating aphid transmission.QD5 and QD6 shared nucleotide identities of 95.3%-99.4% and aa identities of 93.5%-99.6% in cp genes with other PeMoV isolates available in the GenBank.The phylogenetic results showed that PeMoV were clustered to three groups,America,Asia and Australia,which were consistent with their geographical origins.This is the first molecular evidence on the incidence of PeMoV in China.

关 键 词：斑驳病毒 基因序列 花生 分离物 克隆 CP 青岛 自然侵染 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]