云南甘蔗杆状病毒的分子检测及其对甘蔗品质和产量的影响  

Detection of Sugarcane bacilliform virus isolate and its influence on yield and quality of cane in Yunnan

作　　者：李文凤[1,2] 黄应昆 姜冬梅[2] 张志想[2] 张本利 李世访[2] 

机构地区：[1]云南省农业科学院甘蔗研究所云南省甘蔗遗传改良重点实验室,开远661600 [2]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物病理学报》2010年第6期651-654,共4页Acta Phytopathologica Sinica

基　　金：现代农业产业技术体系建设专项资金(nycytx-024-01-09);公益性行业(农业)科研专项(nyhyzx07-019);国家"西部之光"访问学者项目资助(2008年度)

摘　　要：Sugarcane bacilliform virus(SCBV) was detected by PCR from sugarcane showing chlorosis and mottle symptom from Kaiyuan,Yunnan Province.Part sequence of replicase gene of the isolate SCBV-Kaiyuan was determined.Sequence analysis indicated that the 589 bp of SCBV-Kaiyuan shared identities of 73.2%-74.0% and 83.1%-84.1% at nucleotide and amino acid levels with SCBV-Australia respectively,66.7%-68.4% and 65.6%-67.7% with SCBV-Morocco.The quality and yield of the sugarcane infected with SCBV-Kaiyuan was also investigated.The juice extraction,sucrose content,gravity purity and average stalk weight were decreased 1.55%,1.24%,2.22% and 0.26 kg in plants infected with SCBV-Kaiyuan,but reducing sugar was increased by 0.21% in infected plants.Sugarcane bacilliform virus（SCBV） was detected by PCR from sugarcane showing chlorosis and mottle symptom from Kaiyuan,Yunnan Province.Part sequence of replicase gene of the isolate SCBV-Kaiyuan was determined.Sequence analysis indicated that the 589 bp of SCBV-Kaiyuan shared identities of 73.2%-74.0% and 83.1%-84.1% at nucleotide and amino acid levels with SCBV-Australia respectively,66.7%-68.4% and 65.6%-67.7% with SCBV-Morocco.The quality and yield of the sugarcane infected with SCBV-Kaiyuan was also investigated.The juice extraction,sucrose content,gravity purity and average stalk weight were decreased 1.55%,1.24%,2.22% and 0.26 kg in plants infected with SCBV-Kaiyuan,but reducing sugar was increased by 0.21% in infected plants.

关 键 词：甘蔗品种 杆状病毒 分子检测 产量 品质 云南 virus 甘蔗种植区 

分 类 号：S566.1[农业科学—作物学]