采用SNP分型方法对甲醛固定组织进行个体识别  

SNP Typing of Formalin-fixed Tissue in Individual Identification

作　　者：林源[1] 李莉[1] 柳燕[1] 赵珍敏[1] 

机构地区：[1]司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063

出　　处：《法医学杂志》2010年第6期446-448,共3页Journal of Forensic Medicine

基　　金：中央级科研院所社会公益研究专项项目(GY0803)

摘　　要：目的通过对X染色体SNP遗传标记的检测,探讨甲醛固定组织块的DNA分型策略。方法提取甲醛固定组织块的DNA,在用SinofilerTM试剂盒、MiniFilerTM试剂盒检测未能获得分型结果的情形下,采用多重PCR和飞行质谱技术对X染色体上的51个SNP位点进行分型检测。结果对于常染色体STR、miniSTR分型失败的甲醛固定组织块,X-SNP分型获得成功。结论对于甲醛固定组织块等微量、降解的生物学检材,若常染色体STR基因座分型失败,可尝试进行SNP位点的分型,以获得更多的遗传信息。Objective To explore the method for DNA typing in formalin-fixed tissue by detecting SNP markers on X chromosome.Methods Genomic DNA was prepared from formalin-fixed tissue.In the event that typing using SinofilerTM and MiniFilerTM kits had failed,51 SNPs were amplified with multiplex PCR and typed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry（MALDI-TOF MS）.Results Full profiles of X-SNP loci were obtained from the formalin-fixed tissue,while the STR and miniSTR genotyping failed.Conclusion SNP genotyping technique can be used to obtain more information for formalin fixed tissues.

关 键 词：法医遗传学 多态性 单核苷酸 甲醛 个体识别 

分 类 号：DF795.2[医药卫生—法医学]