水稻第6染色体短臂每穗实粒数和每穗颖花数QTL的精细定位  

FINE MAPPING OF A QTL FOR NUMBER OF GRAINS PER PANICLE AND NUMBER OF SPIKELETS PER PANICLE ON SHORT ARM OF RICE CHROMOSOME 6

作　　者：樊叶杨[1] 程式华[1] 范方军[1] 庄杰云[1] 

机构地区：[1]中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室,浙江杭州310006

出　　处：《核农学报》2010年第6期1105-1109,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(30571062);农业部超级稻专项(200906);中央级公益性科研院所基本科研业务费专项资金项目(2009RG002)

摘　　要：对水稻第6染色体短臂上一个控制每穗实粒数和每穗颖花数的QTL进行精细定位研究。针对前期定位的RM587-RM6119区域,应用在目标区间内杂合片段呈交叉排列的3个剩余杂合体,自交后获得3套F2∶3群体,对每穗实粒数、每穗颖花数和单株产量进行QTL分析。在3套群体中均检测到控制每穗实粒数、每穗颖花数和单株产量的QTL,其遗传作用均以加性作用为主,增效等位基因来自母本珍汕97B,且QTL的效应在各群体间相近。因此,该目标区间存在一个共同的QTL,通过控制每穗粒数来调控单株产量。通过比较3个剩余杂合体共有的杂合区间,最终将控制每穗实粒数和每穗颖花数的QTL定位于RM3414-RM19417之间约96.4kb的区域内。A quantitative trait locus（QTL） for number of grains per panicle（NGP） and number of spikelets per panicle（NSP） on the short arm of rice chromosome 6 was fine mapped.Three residual heterozygous lines（RHLs）,which carried overlapping heterozygous segments in interval RM587-RM6119,were selected.Three F2：3 populations were developed and used for QTL analysis for number of grains per panicle,number of spikelets per panicle and grain yield per plant（GY）.The QTL for NGP,NSP and GY were detected in all three populations.The genetic action mode and allelic direction of each QTL were consistent across three populations,showing additive action with the increasing allele from maternal line Zhenshan 97B.The magnitude of additive effects was also similar among three populations.This suggests that a QTL was located in the target interval regulating GY by controlling NGP and NSP.By comparing the common heterozygous regions among three RHLs,the QTL for NGP and NSP was delimited in a 96.4kb-segment flanked by RM3414 and RM19417.

关 键 词：水稻 数量性状座位(QTL) 剩余杂合体 每穗实粒数 每穗颖花数 

分 类 号：S511[农业科学—作物学]