45Ca示踪技术研究三七提取物对细胞Ca2＋内流与外溢的影响  

Ca^(2+) INFLUX AND EFFLUX IN ANIMAL CELLS IN THE PRESENCE OF Panax notoginseng EXTRACTS: INVESTIGATED BY USING ^(45)Ca AS A RADIOACTIVE TRACER

作　　者：杨远友[1] 刘宁[1] 莫尚武[1] 廖家莉[1] 许发伦[1] 

机构地区：[1]四川大学原子核科学技术研究所,辐射物理及技术教育部重点实验室,四川成都610064

出　　处：《核农学报》2010年第6期1305-1308,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(10175044,10975103)

摘　　要：采用45Ca同位素示踪技术,进行了三七提取物对大鼠内脏器官细胞Ca2+内流与外溢的影响研究。结果表明,从三七中提取的总皂苷和总黄酮对大鼠的心脏、肾脏、动脉平滑肌等内脏器官细胞钙通道具有一定的调控作用,既能阻滞Ca2+内流,也能促进已流入细胞内的Ca2+外溢。与总黄酮相比,总皂苷的作用更强,其调控作用可与阳性对照药物Verapamil相比。这一结果提示皂苷和黄酮提取物具有钙拮抗作用,可能是治疗冠心病的有效成分。In this paper,the influence of extracts of Panax notoginseng on Ca2 ＋ influx and efflux in isolated rat visceral organs was investigated by using 45Ca as a radioactive tracer.The results indicated that both extracts,the total flavonoids and total saponins of Panax notoginseng had significant influence on Ca2 ＋ influx and efflux in the isolated rat aorta,heart,and kidney,in those organs it could markedly block 45Ca entering into cell and could facilitate efflux of intracellular Ca2 ＋.Compared with the total flavonoids,total saponins had stronger role in the regulation of Ca2 ＋ influx and efflux.Also,regulation effects of Ca2 ＋ influx and efflux of the total saponins were compared with positive drug Verapamil,or evev better.This implies that the total flavonoids and total saponins of Panax notoginseng have calcium antagonistic effect,and both may be the active ingredients in Panax notoginseng for coronary heart disease treatment.

关 键 词：45Ca 三七 CA2+ 总皂苷 总黄酮 

分 类 号：R285.5[医药卫生—中药学]