共振瑞利散射光谱法测定利血平的含量  

Determination of Reserpine by Resonance Rayleigh Scattering Spectrometry

作　　者：江虹[1] 庞向东[1] 邓钦心[1] 黄建琼[1] 李雪芹[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《理化检验（化学分册）》2010年第12期1428-1430,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：重庆市教委科技基金资助项目(No:KJ101315);长江师范学院科研资助项目(No:2008xky38)

摘　　要：在pH3.96的Tris-盐酸缓冲介质中,利血平的水解产物和质子化的维多利亚蓝B形成离子缔合物,使体系的共振瑞利散射(RRS)急剧增强,在421nm处产生最大散射峰,利血平的质量浓度在0.40～6.40mg·L^(-1)范围内与共振瑞利散射强度(△I_(RRS)=I_(s,RRS)-I_(0,RRS))呈线性关系,检出限(3S_b/k)为18.27μg·L^(-1)。应用此方法测定了市售利血平注射液中利血平含量,并以此试样为基体,用标准加入法做回收率和精密度试验,测得平均回收率为101.0%,相对标准偏差(n=6)为2.1%。In a Tris-HCl buffer medium of pH 3.96,the hydrolysate of reserpine（an alkaloidal drug） was reacted with the protonized victoria blue B to form a hydrophobic ion association complex giving strong signal of resonance Rayleigh scattering（RRS） at the wavelength of 421 nm.Linear relationship between values of RRS intensity（ΔI_（RRS）= I_（s,RRS）—I_（0,RRS）） and mass concentration of reserpine was kept in the range of 0.40 to 6.40 mg·L^（-1）,with value of detection limit（3s_b/k） of 18.27μg·L^（-1）.The proposed method was used to analyze 2 samples of reserpine injections,and tests for recovery and precision were made by standard addition method using these samples as matrixes.Values of average recovery and RSD＇s（n=6） found were 101.0%and 2.1%respectively.

关 键 词：利血平 维多利亚蓝B 共振瑞利散射 

分 类 号：O657.3[理学—分析化学]