反相高效液相色谱法测定花椒的3种黄酮苷元的含量  

RP-HPLC Determination of 3 Flavonoid Aglycones of Zanthoxylum Bungeanum

作　　者：吴亮亮[1,2] 石雪萍[1] 张卫明[1] 

机构地区：[1]南京野生植物综合利用研究院,南京210042 [2]南京农业大学食品科技学院,南京210095

出　　处：《理化检验（化学分册）》2010年第12期1441-1443,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家"十一五"科技支撑计划重点课题(2006BAD06B02)

摘　　要：提出了反相高效液相色谱法测定花椒的3种水解黄酮苷元槲皮素、山柰酚、异鼠李素的含量。采用Zorlbax Eclipse C_(18)色谱柱(4.6mm×150mm,5μm),以甲醇-0.4%(体积分数)磷酸溶液(1+1)混合溶液为流动相,在360nm波长处进行测定。槲皮素的质量浓度在2.19～109.6mg·L^(-1),山柰酚的质量浓度在2.28～114.0mg·L^(-1),异鼠李素的质量浓度在2.80～140.0mg·L^(-1)时分别与其峰面积呈线性关系。槲皮素、山柰酚和异鼠李素的加标回收率分别为95.6%,104.4%,103.8%,相对标准偏差(n=5)分别为2.98%,3.97%,4.30%。RP-HPLC was applied to the determination of 3 flavonoid aglycones,including quercetin, kaempferol and isorhamnetin in zanthoxylum bungeanum.The sample solution was separated by using Zorbax Eclipse C_（18） column（4.6 mm×150 mm,5μm） as stationary phase and a mixture of methanol and 0.4%（φ） phosphoric acid solution（mixed 1 to 1 by vol.） as mobile phase.The contents of the 3 flavonoid aglycones were determined at the wavelength 360 nm.Linear relationships between values of peak area and mass concentration of quercetin,kaempferol and isorhamnetin were kept in ranges of 2.19-109.6 mg·L^（-1）,2.28-114.0 mg·L^（-1） and 2.80-140.0 mg·L^（-1）,respectively.Tests for recovery and precision were made by standard addition method. Values of recovery and RSD＇s（n = 5） found were 95.6%and 2.98%for quercetin,104.4%and 3.97%for kaempferol,103.8%and 4.30%for isorhamnetin,respectively.

关 键 词：反相高效液相色谱法 花椒 槲皮素 山柰酚 异鼠李素 

分 类 号：O652.63[理学—分析化学]