拟南芥atfesla突变体种子的热敏感性分析  

THE HEAT- SENSITIVE ANALYSIS OF THE atfesl a MUTANT SEEDS IN ARABIDOPSIS THALIANA

作　　者：杨颖[1] 杨传燕[1] 刘箭[1] 

机构地区：[1]山东师范大学生命科学学院,济南250014

出　　处：《山东师范大学学报（自然科学版）》2010年第4期138-140,共3页Journal of Shandong Normal University(Natural Science)

基　　金：国家自然科学基金资助项目（30970233）.

摘　　要：根据基因．簖片数据等生物信息学资料，我们锁定一些未知的且被高温诱导的基冈，并获得了一个推测编码蛋白含armadillo／beta—cateninrepeat（简称ARM）结构域的突变体salk-021784，该突变体缺失AtFeslA基因，该基因编码一种受高温诱导的ARM蛋白．利用抗体检测了杠拟南芥种子发育过程中AtFeslA的热诱导表达特征，通过表型分析发现，热处理后的突变体种子萌发率明盟低于热处理后的野生型，表明AtFeslA蛋白与拟南芥耐热有关．By Bioinformatics data like gene chip, we focus on some genes which induced by high -temperature , and we get a new mutant salk -021784. This mutant lost AtFeslA gene is predicted to encode an armadillo/ beta- catenin repeat protein. By the AtFeslA antibody, we detect the expressive characteristics of heat shock of AtFeslA in the course of Arabidopsis thaliana seed development. Through the analysis of the phenotype, we find that the mutant presents a defection of acquired - thermotolerance and its seed germination rate is lower than that of the wild type,which indicates that the AtFesl A protein plays an important role in Arabidopsis thaliana thermotoler- ance.

关 键 词：拟南芥 AtFeslA Western—blotting 热做感表型 

分 类 号：Q78[生物学—分子生物学]