双黄连口服液体外抗单纯疱疹病毒1型的初步探讨  

PRELIMINARY STUDY ON ANTI- HSV- 1 ACTIVITY OF SHUANG HUANG LIAN ORAL LIQUID IN VITRO

作　　者：姜光域[1] 王斌[1] 宋旭霞[1] 

机构地区：[1]青岛大学医学院青岛市医药生物技术重点实验室,山东青岛266071

出　　处：《山东师范大学学报（自然科学版）》2010年第4期141-146,共6页Journal of Shandong Normal University(Natural Science)

基　　金：国家重大基础研究前期专项（2004CCA024000）;青岛市科技专项（05-1-P-10）;山东省科技发展计划项目（2004GG2205136）;泰山学者工程资助.

摘　　要：目的探讨双黄连口服液体外抗单纯疱疹病毒1型（HSV-1）的作用．方法选用非洲绿猴肾细胞VERO作为体外培养靶细胞，建立HSV-1感染，用不同剂量的双黄连口服液处理HSV-1感染细胞，以50％组织细胞感染量（TCDI50），细胞病变效应（CPE）为评测指标；采用MTT法，RT—PCR及流式细胞技术作为检测手段并比较药物处理组与HSV-1感染组细胞闻病毒表达的差异．结果浓度为标准剂量10％的口服液可明显抑制HSV-1gD基因mRNA的转录，选择性抑制HSV-1DNA的合成；在标准药物剂量0．1％-10％范围内病毒感染组细胞与实验组细胞问凋亡呈现差异性．结论双黄连口服液对体外感染的HSV-1型病毒存在抑制作用，显示出一定的抗病毒活性．The anti-HSV-1 activity of Shuang Huang Lian oral liquid was studied. The oral liquid in various concentrations was applied to VERO cells infected by HSV-1. Fifty percent tissue culture infective dose （TCDIs0） , cytopathic effect （CPE）, were used as estimating index for antiviral activity. MTF, RT-PCR, and flow cytometry methods were used as test facility to compare of differences of HSV expression between the experimental group and HSV infected cells. The 10% concentration of standard oral liquid can be shown obviously inhibitory effects on HSV-1 gD mRNA expression, it can selectively inhibit the viral DNA synthesis. It shows differences in cell apopto- sis between experimental group and HSV infected cells. Shuang Huang Lian oral liquid possesses a clearly anti- HSV-1 function in vitro. It can inhibit the activity of HSV-1.

关 键 词：双黄连口服液 绿猴肾细胞(VERO) 单纯疱疹病毒1型(HSV一1) 抗病毒 

分 类 号：R931.77[医药卫生—生药学]