Quantification of eugenol and bancroftione in Caryophylli Fructus using high-performance liquid chromatography  

HPLC法测定母丁香药材中丁香酚和母丁香酚的含量(英文)

作　　者：刘淑娟[1] 林宗涛[1] 王弘[1] 陈世忠[1] 

机构地区：[1]北京大学医学部药学院天然药物学系,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》2010年第6期459-463,共5页中国药学（英文版）

基　　金：973 Project-Medical Characteristics of Orthodox Herbs(Grant No.2006CB504707)

摘　　要：For the first time, we have utilized high-performance liquid chromatography （HPLC） to simultaneously quantify the eugenol and bancroffione in Caryophylli Fructus. The optimized parameters included： Inertsil ODS-4 column （150 mm×4.6 mm, 5 μm）; column temperature： 35 ℃; mobile phase： methanol water （65：35, v/v）; flow rate： 1.0 mL/min; detection wavelength： 280 nm. Eugenol and bancroftione showed good linear relationships with peak areas within the range of （0.0998 0.8982） mg/mL （r = 0.9999） and （0.1474-1.3266） mg/mL （r = 0.9999）, respectively. The average recoveries were 102.52% and 100.96% for eugenol and bancroftione, respectively. Our results showed that the established method is simple, rapid, and accurate with good reproducibility to evaluate the quality of Caryophylli Fructus.建立母丁香中丁香酚和2-羟基-4,6-二甲氧基-5-甲基苯乙酮(母丁香酚,bancroftione)的HPLC含量测定方法。采Inertsil ODS-4色谱柱(4.6mm×150mm,5μm);柱温35℃;流动相为甲醇-水(65:35,v/v);流速1.0mL/min;检测波长280nm。丁香酚和母丁香酚分别在0.0998～0.8982mg/mL(r=0.9999)和0.1474～1.3266mg/mL(r=0.9999)范围内与峰面积呈良好的线性关系。平均加样回收率分别为102.52%和100.96%。本文建立了母丁香药材中丁香酚和母丁香酚含量测定方法,具有简便、快速、准确、重现性好等优点,可用于母丁香药材的质量评价。

关 键 词：Caryophylli Fructus EUGENOL Bancroftione Quantification HPLC 

分 类 号：R284.1[医药卫生—中药学]