抗肝衰复方对急性肝衰竭大鼠的防护作用及对肿瘤坏死因子-α表达的影响  

Effects of Kangganshuai Fufang on protective function and expression of TNF-alpha in rats with acute hepatic failure

作　　者：张建军 艾陈芳[2] 戴丹 

机构地区：[1]湖北省中山医院感染科,湖北武汉430033 [2]国家食品药品监督管理局

出　　处：《中西医结合肝病杂志》2010年第6期353-355,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:观察抗肝衰复方(KGSFF)对急性肝衰竭大鼠防护作用及肿瘤坏死因子(TNF-α)表达的影响,探讨KGSFF对大鼠肝衰竭的作用机制。方法:健康雄性Wistar大鼠110只,随机分为正常对照组10只,模型组20只、促肝细胞生长素(PHGF)阳性对照组20只、茵陈蒿汤(YCHT)组20只、KGSFF小剂量组20只、KGSFF大剂量组20只。除正常对照组外其余各组大鼠分别给予D-氨基半乳糖(D-GalN)800mg/kg和内毒素脂多糖(LPS)0.1mg/kg的剂量,腹腔注射1次成模,正常对照组大鼠给予等量生理盐水腹腔注射。除模型组和正常对照组外各组大鼠均于造模后2小时用药,按每次1.5ml/100g,每天灌胃两次。模型组和正常对照组大鼠均分别给予生理盐水灌服。造模后72小时取材。观察造模后72小时内大鼠病死率。检测血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、总胆红素(TBil)及肿瘤坏死因子-α(TNF-α)水平。HE染色观察肝组织病理变化,免疫组织化学染色法检测肝组织TNF-α水平。结果:KGSFF大剂量组大鼠病死率及血清ALT、AST、TBil含量降低,肝细胞损伤程度显著改善,血清及肝组织TNF-α水平明显降低,与模型组相比P<0.01或P<0.05。结论:KGSFF对急性肝衰竭大鼠具有明显的防护作用,其机制可能与抑制TNF-α产生,降低内毒素血症,改善肝功能有关。Objective：To observe the effects of Kangganshuai Fufang（KGSFF）on the protective function and the expression of TNF-α and to explore its mechanism related to relieve the damage and promoting regeneration in rats with laboratory acute hepatic failure.Methods：One hundred and ten Wistar male rats were randomly divided into six groups： normal control group（n=10）,model control group（n=20）,PHGF control group（n=20）,YCHT group（n=20）,KGSFF group of small dose（n=20）,KGSFF group of large dose（n=20）.All rats were injected with D-GalN 800mg/kg and LPS 0.1mg/kg except the normal group injected with the salt solution intraperitoneally.In 2 hours after model being made,the rats of nomal control group and model control group were treated with salt solution,KGSFF low and high dosages,YCHT,PHGF were treated respectively at a dosage of 1.5ml/100g twice every day for 3 days.And 72 hours later,the blood and hepatic tissues were collected.The mortality of the rats were observed in 3 days.The liver function including serum level of ALT,AST,TBil and TNF-α were measured by biochemical methods.The pathological morphologies of hepatic tissues were examined with HE staining.The level of TNF-α in hepatic tissues were measured by immunohistochemical staining.Results：Compared with those in the model rats,the mortality of the rats and serum ALT,AST,TBil and TNF-α level remarkablely decreased,the cell necrosis infiltration were significantly improved,and the level of TNF-α in hepatic tissues also droped in the KGSFF group of large dose（P0.01 or P0.05）.Conclusion：The KGSFF has an outstanding protective function on the rats with acute hepatic failure,and its mechanism may be related to decreasing TNF-α,relieving the intestinal endotoxemia and improving the liver function.

关 键 词：抗肝衰复方/药效学 急性肝衰竭 肿瘤坏死因子-Α 大鼠 

分 类 号：R285.5[医药卫生—中药学]