6%抗坏血酸水剂抗烟草马铃薯Y病毒的作用机制  

Action Mechanism of 6% Ascorbic Acid Solution against Potato Virus Y of Tobacco Leaves

作　　者：蔡学建[1] 向章敏[2] 李家俊 吴英剑 熊茂荣 尹俊生 韩中明 黄荣茂[1] 

机构地区：[1]赣州有色冶金研究所,江西赣州341000 [2]贵州省烟草科学研究所,贵州贵阳550081 [3]贵州省烟草公司,贵州贵阳550036 [4]贵州省烟草公司黔南州烟草分公司,贵州都匀558000

出　　处：《贵州农业科学》2010年第12期106-108,共3页Guizhou Agricultural Sciences

基　　金：国家科技成果重点推广计划项目(2004EC000313-040452)

摘　　要：为了解6%抗坏血酸水剂对PVY的室内活性抑制效果,采用半叶枯斑法及常规汁液摩擦方法,测定了6%抗坏血酸水剂抗PVY的生物活性,检测了施用6%抗坏血酸水剂后接种PVY的烟草叶片中丙二醛、脯氨酸、可溶性糖、可溶性蛋白及硝酸还原酶等相关生理指标的变化。结果表明:6%抗坏血酸水剂对烟草有较高的抗PVY保护活性,施用6%抗坏血酸水剂后接种PVY烟草叶片中的丙二醛、脯氨酸、可溶性糖、可溶性蛋白等重要化学物质的含量变化呈现有利于烟草品质的提高;硝酸还原酶的活性均较对照处理的高。6%抗坏血酸水剂能够诱导烟草产生对PVY的抗性并对感染PVY的烟草起到保护作用。The inhibition effect of 6% ascorbic acid solution on PVY activity was determined by the half-leaf and conventional juice friction methods and the content of MDA,praline,soluble sugar,soluble protein and nitrate reductase activity in tobacco leaves inoculated with PVY were determined after spraying 6% ascorbic acid solution to study the inhibition effect on PVY activity in door and effect on related physiological indexes of tobacco leaves.The results showed that 6% ascorbic acid solution could effectively improve resistance to PVY,changes of MDA,praline,soluble sugar and soluble protein content in tobacco leaves inoculated with PVY after spraying 6% ascorbic acid solution was beneficial to improvement of tobacco quality,and its nitrate reductase activity was higher than CK.Therefore,6% ascorbic acid solution can induces tobacco resistance to PVY and has the protection effect on tobacco infected with PVY.

关 键 词：6%抗坏血酸水剂 马铃薯Y病毒 烟草 作用机制 

分 类 号：S532[农业科学—作物学]