大孔吸附树脂分离纯化柚皮总黄酮的研究  

Purification Process of Flavonoids from the Skin of Citrus Grandis (L.) Osbeck with Mmacroreticular Resin

作　　者：罗文敏 陈新美[2] 陈会明 贾强[1,3] 

机构地区：[1]贵州省生物研究所,贵阳550009 [2]广州医学院,广州510275 [3]贵州科学院贝科生物资源研究开发中心,贵阳550009

出　　处：《贵州科学》2010年第4期83-87,共5页Guizhou Science

基　　金：贵州省农业攻关项目(黔科合农G字[2009]4013);贵州省自然基金(黔科合J字[2009]2290);贵州省委组织部高层次人才基金(TZJF-2009-45);贵州省农业重点攻关项目(黔科合NY字[2009]3021);贵州科学院人才引进项目(黔科院人合字[2009]01)

摘　　要：通过研究大孔吸附树脂分离纯化柚皮总黄酮的工艺,为柚皮总黄酮的工业化生产提供实验依据。本文以广东产柚皮为原料,以柚皮总黄酮含量及回收率等为考察指标,选用大孔吸附树脂对柚皮总黄酮进行分离纯化,分别采用静态试验、动态试验等分别考察大孔树脂对柚皮总黄酮的分离纯化效果及影响因素。结果表明,D101型大孔吸附树脂对柚皮总黄酮静态饱和比吸附量为63.76mg.g-1(干树脂),洗脱率99.37%,纯度在51.56%以上,是实验树脂中分离纯化柚皮总黄酮的最佳大孔吸附树脂。因此采用本法分离纯化柚皮总黄酮是稳定、高效的,可进一步推广应用于工业化生产。Based on a method for separation and purification of flavonoids from the skin of citrus grandis （L.） Osbeck with macroporous resin,the study provided experimental evidence for industrial production of flavonoids from the skin of citrus grandis （L.） Osbeck. By using the skin of citrus Grandis （L.） Osbeck from Guangzhou as material with content and recovery rate of flavonoids as indexes,the static and dynamic adsorption tests were employed to investigate effects and affective factors of separation and purification of flavonoids from the skin of citrus grandis （L.） Osbeck with macroporous resin. Results showed that the static adsorption capacity of D101 type resin was 63.76 mg·g-1,the recovery rate was more than 99.73% and the purity of flavonoids was more than 51.56 %. D101 type resin was the best for separation and purification of the skin of citrus grandis （L.） Osbeck in flavonoids. Therefore,the technology of separation and purification of flavonoids from the skin of citrus grandis （L.） Osbeck with D101 type resin was stable and effective,and could be further applied to industrial manufacturing.

关 键 词：大孔树脂 柚皮 总黄酮 分离 纯化 

分 类 号：R943[医药卫生—药剂学]