连翘授粉受精过程的观察  

Observation of Pollination and Fertilization Process in Forsythia suspensa

作　　者：刘红霞[1] 任士福[1] 张成合[1] 刘铭 

机构地区：[1]河北农业大学,保定071001 [2]石家庄中药材研究所,石家庄050051

出　　处：《林业科学》2010年第9期45-49,共5页Scientia Silvae Sinicae

基　　金：国家发改委中药材扶持项目(2006BAI06A13-03);河北省教育厅项目(2007464)

摘　　要：为探索连翘自然结果率较低的原因,以长花柱和短花柱2种类型的连翘为材料,采用荧光显微和石蜡切片技术,对自花授粉和杂交授粉的授粉受精过程进行研究。结果表明:在自花授粉的情况下,授粉后48h,长花柱连翘的花粉管刚开始进入花柱,短花柱连翘的花粉管已到达花柱的中部,但此后花粉管生长速度均很慢,仅后者有极少数胚珠受精,前者则未见胚珠受精的现象;在2种类型连翘相互授粉的情况下,以长花柱连翘为母本,授粉后168h花粉管进入胚珠,以短花柱连翘为母本,授粉后120h花粉管即进入胚珠,但二者完成受精作用均在授粉后18天左右。Forsythia suspensa is an important Chinese medicine plant,but its bearing rate is low naturally.In order to explore the reason of low capsule setting,the long and short style type of Forsythia suspensa were used for observing pollination and fertilization in self-pollination and cross-pollination by fluorescence microscopy and paraffin slice techniques.The results showed as follows：under the condition of self-pollination,a few of pollen tubes could enter the style canal in long and short style F.suspensa in 48 h after pollination,but no fertilized ovule was observed ultimately in the former and few of fertilized ovule existed in the latter.Under the condition of cross-pollination between the long and short style F.suspensa,the number of pollen tube was more and the growth was faster than that of self-pollination.The pollen tube growing into the ovule took about 168 h after pollination in the long style and about 120 h in the short one.It needed about 18 days to accomplish the fertilization in the both style type.

关 键 词：连翘 授粉 受精 花粉管 花粉 

分 类 号：S718.47[农业科学—林学]