黑龙江沙棘干缩病病原菌的研究  

Research on the Pathogen of Dry Shrink on Seabuckthorn(Hippophae rhamnoides) in Heilongjiang Province

作　　者：宋瑞清[1,2] 孙海珍[1] 董希文[3] 邓勋[1,2] 

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]黑龙江省林业科学院,哈尔滨150081 [3]黑龙江省森林与环境科学研究院,齐齐哈尔161005

出　　处：《林业科学》2010年第9期88-95,共8页Scientia Silvae Sinicae

基　　金：黑龙江省"十一五"科技攻关项目(GB06B304-6)

摘　　要：2006—2007年分秋、春、夏3次采集大果沙棘干缩病感病植株感病部位组织,分离纯化、鉴定获得8个真菌菌株。根据Koch's证病法则,选择分离自具有明显干缩病症状植株感病部位的分离物的孢子作为致病性测定用接种体,分别采用菌剂灌根、干部刮伤涂抹菌剂的方法对沙棘幼苗和12年生沙棘进行接种后,引起与自然发病相一致的症状,对接种发病的植株再分离获得3个菌株。对上述菌株进行形态学及分子生物学测定,将其鉴定为拟枝孢镰孢菌。该病原菌可生长在沙棘的叶部、枝干部及根部,生长在不同部位的菌株存在致病力上的差异。该病原菌在大果沙棘上的危害在黑龙江省尚属首次报道。Eight fungal strains were isolated from infested parts of diseased seabuckthorn plants,collected at autumn,spring and summer from 2006 to 2007.According to Koch＇s postulates,the spores of isolates from the infested parts were used as inoculums for inoculating seedlings and 12-year-old seabuckthorn plants.The same disease symptoms were reproduced with isolates.Three strains were recovered after Koch test.Original isolates and recovered strains were identified as Fusarium sporotrichioides based on morphological characteristics and 18S rDNA sequence analysis（100% nucleotide identity）.The pathogen was able to grow on or in the leaf,branch,trunk and root of seabuckthorn plants,and there was difference in pathogenicity between the pathogen strains which grow on or in different parts of plant.This is the first report that F.sporotrichioides is the pathogen of seabuckthorn dry shrink in Heilongjiang Province.

关 键 词：大果沙棘 干缩病 病原鉴定 拟枝孢镰孢菌 

分 类 号：S763.15[农业科学—森林保护学]