荧光增白剂、寄主植物、LdNPV地理品系对舞毒蛾幼虫酚氧化酶的影响  

Effect of a Fluorescent Brightener,Host Plants and Geographic Strains of LdNPV on the Phenoloxidase Activity of Gypsy Moth Larvae

作　　者：杨高鹏[1] 段立清[1] 宫玉艳[1] 杨帆[1] 孙彦宏[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《林业科学》2010年第9期105-109,共5页Scientia Silvae Sinicae

基　　金：加拿大太平洋森林中心合作项目[MPBI-01(2003-05)]

摘　　要：为探索荧光增白剂Tinopal LPW对舞毒蛾核型多角体病毒(LdNPV)的增效机制,明确寄主植物、LdNPV地理品系对舞毒蛾酚氧化酶(Phenoloxidase,PO)的影响,用Tinopal LPW,LdNPV3种不同地理品系(LdNPV-H品系、LdNPV-D品系、LdNPV-J品系)以及它们的混合液共6个处理,蒸馏水及Tinopal LPW2个对照,分别处理以青杨、华北落叶松和山杏为寄主植物的舞毒蛾5龄幼虫。在处理后12,24,48,72,96h测定舞毒蛾5龄幼虫血淋巴酚氧化酶(PO)比活力,每处理30头5龄幼虫,重复3次。结果表明:寄主植物对舞毒蛾5龄幼虫血淋巴PO比活力的影响差异显著。纯病毒品系感染舞毒蛾幼虫时,12h取食3种植物的幼虫血淋巴PO比活力均高于对照,24h后低于对照。3个地理品系与Tinopal LPW混合液感染舞毒蛾幼虫,其PO活力与取食的寄主植物有关,取食山杏时,3个品系的Tinopal LPW混合液对PO活力的抑制均显著高于纯病毒品系及对照。取食青杨或华北落叶松时,H品系和D品系的Tinopal LPW混合液对PO活力的抑制显著高于单独病毒品系,但J品系的Tinopal LPW混合液对PO活力的抑制与单纯J品系无显著差异。Tinopal LPW对照与清水对照比较,显著降低了舞毒蛾幼虫PO活力。To explore the mechanism of a fluorescent brightener,Tinopal LPW,in enhancing infection of LdNPV and the effect of host plants and LdNPV geographic stains on Phenoloxidase in gypsy moths,the 5th instar larvae of gypsy moth,which are pests of Populus cathayana,Larix principis-rupprechtii and Aarmeniaca sibirica were treated with 3 virus strains（LdNPV-D,LdNPV-H,LdNPV-J） and their mixture with Tinopal LPW with and without Tinopal LPW,respectively.Incubation with distilled water or Tinopal LPW only served as controls.As a result,there were 6 treatments and 2 controls.Phenoloxidase（PO） activity in haemolymph of larvae was measured at 12,24,48,72 and 96 h after the treatments.The experiment（30 larvae per treatment） was replicated three times.The results showed that effects of host plants,virus strain and the brightener on the PO activity in the 5th instar gypsy moth larvae were significant.PO activity increased after the larva were infected by pure virus 12 h later,and then decreased after 24 h comparing with both distilled water and Tinopal LPW controls.The effect of D-strain,H-strain and J-strain with addition of Tinopal LPW on the phenoloxidase activity of gypsy moth was dependent on the host plants.Larvae fed on A.sibirica,the PO activity was lower than that in larvae treated with virus strains without the brightener,as well as both controls.Larvae fed on poplar or larch,the PO activity in larvae treated with D-strain,or H-strain with the brightener was lower than that in larvae treated with the two stains only,or controls,but there was no difference in Po activity in larvae treated with J-strain between with and without Tinopal LPW addition.Tinopal LPW significantly reduced the PO activity in comparison with distilled water.

关 键 词：舞毒蛾 LdNPV 酚氧化酶 荧光增白剂 寄主植物 

分 类 号：Q969.437[生物学—昆虫学]