一株拮抗细菌的鉴定及其抗菌特性  

Identification and Antibacterial Characteristic of an Antagonistic Bacterium Strain

作　　者：刘会香[1] 周翠[1] 金静[2] 胡凌志 曹帮华[4] 

机构地区：[1]山东农业大学植物保护学院 [2]青岛农业大学农学与植物保护学院 [3]青岛园林实业总公司 [4]山东农业大学林学院

出　　处：《林业科学》2010年第9期110-114,共5页Scientia Silvae Sinicae

基　　金：山东省优秀中青年奖励基金(2007BS06018);国家自然科技资源共享平台项目"林业微生物菌种资源标准化整理;整合及共享试点"(2005DKA21207-19)部分研究内容

摘　　要：从石榴果实中发现一株拮抗细菌,通过对菌体的形态观察、生理生化特性及16SrDNA序列测定鉴定为枯草芽孢杆菌(Bacillus subtilis);其抗菌特性研究表明:该拮抗菌对几种不同林果病原菌均有不同程度的抑制作用。由Botryosphaeria dothidea、B.parva和Zythia versoniana引起的林果病菌菌丝形态变化表现为菌丝分支增多、扭曲,部分菌丝出现断裂和细胞出现原生质浓缩现象,菌丝顶端和中部细胞膨大成球形或椭圆形,有的成念珠状,有的顶端膨大明显聚集成簇并且颜色加深,随着细胞的继续膨大,透明度增加,最后菌丝细胞变成空泡;而由Valsasordida引起的杨树腐烂病菌菌丝形态变化则表现为菌丝细弱,分支减少,菌丝顶端不明显,不膨大成球形,也不形成念珠状,有的菌丝消解并难以辨认,说明拮抗菌对不同病原菌的抗性机制是不同的。An antagonistic bacterium was found in pomegranate fruit and identified as Bacillus subtilis according to its morphology,physiological and biochemical characteristic and 16S rDNA molecular sequence.Antagonistic characteristics displayed that this strain was able to obviously inhibit growth of eight pathogens.For plant pathogenic fungi of Botryosphaeria dothidea,B.parva and Zythia versoniana,which infect fruits of different trees,the antagonistic bacterium increased mycelia to branch and caused some mycelial cells to distort and fracture and protoplasm to condense.With the bacterium infected,the top and middle mycelial cells intumescentiaed into sphere and oval or bead-like,some intumescentiaed mycelial top cells obviously clustered together and the color became dark,with the mycelial cell intumescentiaed,the cells turned more transparent and changed into vacuole at last.But for Valsa sordida pathogen causing poplar rot disease,the antagonistic bacterium caused mycelia cells to become slim and fragile and less branch.There was no obvious mycelial tops and the cells did not intumescentia into sphere or bead-like,some mycelia were digested and hard to be recognized.All the above results demonstrated that the antagonistic bacterium had different acting mechanism to different causal pathogens.

关 键 词：石榴果实 拮抗菌 鉴定 抗菌特性 作用机制 

分 类 号：S763.13[农业科学—森林保护学]