两株鸭源H1N3亚型流感病毒的分离鉴定和遗传进化分析  

ISOALTION AND CHARACTERIZATION OF H1N3 INFLUENZA VIRUSES OF DUCKS ORIGIN

作　　者：赵国[1] 赵坤坤[1] 鹿欣伦[1] 陈朝阳[1] 王晓泉[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《中国动物传染病学报》2010年第5期1-6,共6页Chinese Journal of Animal Infectious Diseases

基　　金：国家科技重大专项课题(2008ZX10004-013);国家蛋鸡产业技术体系(nycytx-41-G07)

摘　　要：从2008年10月至2010年7月,每月定期在华东地区扬州活禽市场采集健康家鸭泄殖腔拭子,并从中分离鉴定了14株H1亚型禽流感病毒。对其中的2株病毒进行全基因测序和遗传演化分析,发现2株病毒均属于H1N3低致病性禽流感病毒。各基因遗传进化分析均与类禽猪流感的分离株的亲缘关系相对较近;除血凝素(haemagglutini,HA)基因外,其它所有的基因均发生了不同程度基因重组。其中H5N1病毒参与了多聚酶蛋白PB1与PB2基因的重组,H9N2病毒参与了PA基因的重组。Cloacal swab samples were collected monthly from October 2008 to July 2010 from ducks at the live-bird markets.A total of 14 strains of H1 Avian influenza viruses were isolated.The complete gene sequencing and genetic evolution analysis showed that two H1N3 avian influenza viruses possessed low pathogenicity and high genetic homology with avian-like influenza viruses isolated from swine.Except haemagglutini（HA） genes,various degrees of genetic recombinations occurred in other genes.The PB1 and PB2 genes of these strains recombined with those of H5N1 viruses while the PA gene recombined with that of H9N2 viruses.

关 键 词：禽流感病毒 H1N3亚型 基因分析 

分 类 号：S858.322.659.5[农业科学—临床兽医学]