BDE-47的两种代谢产物对HepG2细胞中谷胱甘肽含量和P450酶活性的影响  

Effects of 5-hydroxylated/5-methoxylated-2,2',4,4'-tetrabromodiphenyl Ether on Glutathione Content and Activity of Cytochrome P450 in HepG2 Cells

作　　者：尹灵灵[1] 王流林[1] 钟玉芳[1] 安静[1] 吴明红[1] 盛国英[1] 傅家谟[1] 

机构地区：[1]上海大学环境与化学工程学院,上海200444

出　　处：《上海大学学报（自然科学版）》2010年第6期587-591,共5页Journal of Shanghai University:Natural Science Edition

基　　金：国家重点基础研究发展计划(973计划)资助项目(2008CB41820);上海市重点学科建设资助项目(S30109)

摘　　要：从细胞毒性、氧化应激以及代谢酶活性等方面,研究2,2′,4,4'-四溴联苯醚(2,2′,4,4'-tetrabromodiphenylether,BDE-47)的两种代谢物5-羟基-四溴联苯醚(5-OH-BDE-47)和5-甲氧基-四溴联苯醚(5-MeO-BDE-47)对人体肝癌细胞HepG2的细胞毒性效应.采用噻唑蓝(methyl thiazolyl tetrazolium,MTT)法,检测5-OH-BDE-47和5-MeO-BDE-47染毒后HepG2细胞的生长情况,以及急性染毒后细胞内谷胱甘肽(GSH)含量及细胞内P450酶活性的变化.研究结果表明,5-OH-BDE-47和5-MeO-BDE-47均能抑制HepG2细胞生长,降低GSH活性,诱导EROD和PROD活性的增强.HepG2 cells have been used to study the possible cytotoxicity, the level of glutathione （GSH） and the activity of P450 enzymes after exposure to 5-OH-BDE-47 and 5-MeO-BDE-47. HepG2 cells proliferation was detected after 24 h, 48 h and 72 h exposure with methyl thiazolyl tetrazolium （MTY） test, GSH were measured after cells were treated with 5-OH/5-MeO-BDE47 as above-mentioned doses for 24 h, P450 enzymes were tested after 48 h. After 72 h exposure to 5-OH-BDE-47 and 5-MeO-BDE-47, proliferation of HepG2 cells was inhibited. The content of GSH can be reduced by 5, 10, 20 p^mol/L 5- OH-BDE-47 and only 20 trmol/L 5-MeO-BDE-47. Furthermore, PROD activity was induced by 5-OH- BDE-47 and 5-MeO-BDE-47 under all concentrations, and EROD activity was induced only under higher concentration （20 I^mol/L） of 5-OH-BDE-47 and 5-MeO-BDE-47. Neither 5-OH-BDE-47 nor 5-MeO- BDE-47 was capable of inducing MROD activity. These results show that both 5-OH-BDE-47 and 5-MeO- BDE-47 can inhibit HepG2 cells proliferation, reduce the level of GSH, and increase EROD and PROD activity

关 键 词：多溴联苯醚 2 2’ 4 4’-四溴联苯醚 肝癌细胞 谷胱甘肽 细胞色素 

分 类 号：R994[医药卫生—毒理学]