微丝相关蛋白HSPC300/hHBRK1与肌球蛋白Ⅵ相互作用位点的计算机预测  

Computer Prediction of HSPC300/hHBRK1 Protein and Myosin VI Protein Interaction Sites

作　　者：王飞飞[1] 薛晓怡[1] 王翼飞[1] 

机构地区：[1]上海大学理学院,上海200444

出　　处：《上海大学学报（自然科学版）》2010年第6期635-638,648,共5页Journal of Shanghai University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30871341);上海市重点学科建设资助项目(S30104);上海市教委重点学科建设资助项目(J50101)

摘　　要：HSPC300/hHBRK1蛋白作为BRK1的人类同源蛋白,参与细胞运动和物质运输.肌球蛋白Ⅵ是肌球蛋白家族的成员.HSPC300/hHBRK1和肌球蛋白Ⅵ共同定位于细胞的胞浆,hHBRK1可能通过与肌球蛋白Ⅵ相互作用,参与细胞内的物质运输.从结构角度出发,通过计算机模拟蛋白质间相互作用,预测二者作用区域,找出相关残基PHE43,LYS105,THR676和LEU40,即可能成为相互作用位点的重要残基,为进一步实验研究提供了重要信息.HSPC300/hHBRK1 protein involved in cell movement and material transport is a kind of human homologous proteins. Myosin VI protein is a member of the myosin family. It is possible that HSPC300/hHBRK1 protein and myosin Wl protein react for the reason that they are present in the cytoplasm of cells and they are both involved in intracellular transport of the material. To lay a foundation for further experiments, we predict the area of interaction under the structural point by computer simulation of protein-protein interactions, and find the related residues that may become the sites of amino acid interaction： PHE43, LYS105, THR676 and LEU40.

关 键 词：HSPC300/hHBRKl 肌球蛋白VI 结构预测 分子间对接 

分 类 号：Q81[生物学—生物工程]