献血员感染HBV窗口期的病毒核酸筛查  

Study on NAT assay for the window phase of blood donors' infection with HBV

作　　者：荆永正[1] 孙凤英 于学英 

机构地区：[1]青岛大学医学院,山东青岛266021 [2]蓬莱市人民医院,山东蓬莱265600

出　　处：《实用医药杂志》2010年第12期1064-1066,共3页Practical Journal of Medicine & Pharmacy

基　　金：山东省科学技术发展基金资助(012130118)

摘　　要：目的探讨实时荧光定量PCR技术在临床输血安全领域研究的意义以及我国目前输血残留感染HBV风险,进一步提高临床输血安全性。方法采用实时荧光定量聚合酶链反应(PCR)技术定量检测乙型肝炎表面抗原(HBsAg)阴性献血员血浆中乙型肝炎病毒(HBV)DNA。结果 11456份HBsAg献血员血浆中有41份(0.358%)HBVDNA阳性;对其中34份HBVDNA例阳性标本于3-6月后进行随访检测,有4例HBsAg呈阳性,4例HBVDNA仍为阳性。结论目前我国存在输血残留感染HBV的风险,ELISA技术和荧光定量PCR检测技术在输血领域对HBV检测存在技术互补性。HBVDNA检测可以缩短献血员感染HBV窗口期,提高临床输血的安全性。Objective To explore the real-time quantitative polymerase chain reaction （PCR） in the field of clinical blood transfusion safety and evaluate the risk of HBV residual infection in China for further improving the safety of clinical blood transfusion. Methods The HBV DNA in the plasma of HBsAg negative blood donors was detected by the real-time quantitative PCR. Results The forty-one out of 11 456 （0.358%） HBsAg negative blood donors were HBV DNA positive; Follow-up testing was taken to detect the 34 HBV DNA positive specimen after 3-6 months, 4 were HBsAg positive, 4 were HBV DNA positive. Conclusion At present the risk of HBV residual infection in china still exists. ELISA and real-time quantitative PCR have technical complementarity. The detection of HBV DNA can shorten the window period of HBV infection in blood donors and improve the safety of clinical blood transfusion. period

关 键 词：乙型肝炎病毒 献血员 核酸扩增检测 窗口期 

分 类 号：R392.11[医药卫生—免疫学] R512.62[医药卫生—基础医学]