卵母细胞体外培养时间对单精子胞浆内注射临床结局的影响  

Impact of oocyte preincubation time on clinical outcomes after intracytoplasmic sperm injection

作　　者：顾亦凡[1] 陆长富[1] 龚斐[1] 林戈[1] 卢光琇[1] 

机构地区：[1]中南大学生殖与干细胞工程研究所,湖南长沙410078

出　　处：《中国现代医学杂志》2010年第22期3364-3367,共4页China Journal of Modern Medicine

基　　金：国家重点基础研究发展计划("973"计划)(No:2007CB948103)

摘　　要：目的探索最适合的单精子胞浆内注射(ICSI)时机。方法根据从患者取卵结束到开始ICSI的时间(t),将患者分为3组:A组(2h≤t<4h,n=60),B组(4h≤t≤6h,n=85),C组(6h<t≤8h,n=62)。回顾性分析了卵母细胞体外培养时间对于ICSI患者的受精、胚胎发育及临床结局的影响。结果 B组及C组患者的临床妊娠率及植入率均显著高于A组。结论适度的卵母细胞体外培养时间(4～8h)有利于改善ICSI患者的临床结局。【Objective】To determine the optimal timing of intracytoplasmic sperm injection （ICSI）.【Methods】 According to the oocyte preincubation time （t） between oocyte retrieval and ICSI,patients were assigned into three groups： Group A （2 h≤t 4 h,n =60）,Group B （4 h≤t ≤6 h,n =85） and Group C （6 ht ≤8 h,n =62）.Impact of oocyte preincubation time on fertilization,embryo development and clinical outcomes after intracytoplasmic sperm injection was analyzed retrospectively.【Result】Clinical pregnancy rates and implantation rates in Group B and Group C were significantly higher than Group A.【Conclusion】Appropriate preincubation time could improve clinical outcomes after ICSI.

关 键 词：卵母细胞 体外培养 单精子胞浆内注射 胚胎发育 妊娠 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]