利用单孢子囊接种技术建立鸡柔嫩艾美耳球虫克隆  

Establishment of E.tenella clones by single sporocyst

作　　者：魏峰[1,2] 许越[1] 张西臣[2] 蔡亚男[2] 

机构地区：[1]吉林农业大学生命科学院,吉林长春130118 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2010年第12期1610-1613,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30170696)

摘　　要：利用显微操作从柔嫩艾美耳球虫长春株、河北株、山东株(由单卵囊接种建立)挑选出单孢子囊接种于雏鸡,接种成功率为17.8%。传代后提取DNA,用7条随机引物PCR扩增,鉴别RAPD图谱及特异条带,结果获得柔嫩艾美耳球虫长春株2个克隆、河北株2个克隆、山东株1个克隆。A single sporocyst selected from Changchun,Hebei and Shandong strains of Eimeria tenella respectively,using micromanipulation,innoculated chickens and the success rate was 17.8%.The selected clones were studied by random amplified polymorphic DNA（RAPD） with 7 screened random primers,and identited by the RAPD map and the especial fragment of DNA.The 2,2 and 1 clones of Changchun,Hebei and Shandong strains of E.tenella were obtained,respectively.

关 键 词：鸡 柔嫩艾美耳球虫 单孢子囊 RAPD 

分 类 号：S852.723[农业科学—基础兽医学]