HPLC法测定不同产地黄蜀葵花中黄酮类成分含量  

Determianation of Hyperoside,Myricetin and Quercitin in Flos Abelmoschus corolla by HPLC

作　　者：蒋建春[1] 朱华云[1] 

机构地区：[1]江苏苏中药业集团股份有限公司,泰州225500

出　　处：《药学与临床研究》2010年第6期537-539,共3页Pharmaceutical and Clinical Research

摘　　要：目的:研究建立同时测定黄蜀葵花中多个黄酮成分含量的方法,并比较江苏不同产地黄蜀葵花中黄酮成分的含量差异。方法:采用HPLC法,以金丝桃苷、杨梅素和槲皮素3种黄酮为对照品,以乙腈-0.4%磷酸溶液为流动相,梯度洗脱,在360nm波长下检测,柱温:30℃,流速:1.0mL.min-1。结果:对3种黄酮的方法学考察结果表明符合含量测定要求,三者在一定浓度范围内有良好的线性关系。结论:方法操作简便,结果可靠,重复性好,专属性强,可用于黄蜀葵花药材的质量控制。江苏境内黄蜀葵花中3种黄酮成分含量稍有差异。Objective： To establish a method for the determination of flavones in the flowers of Abelmoschus corolla from different areas of Jiangsu,and compare their content differences.Methods： HPLC was used with hyperoside,myricetin and quercitin as reference substances.The mobile phase was composed with acetonitrile-0.4% phosphoric acid.Gradient elution was applied.The detection wavelength was 360 nm.The column temperature was 30℃.The flow rate was 1.0 mL·min-1.Results： The results of the method inspection of the three flavones met the determination requirements with linearity at respective range.Conclusion： This method is simple,rapid and accurate.It can be used for quality control of the drug.The contents of the three flavonoids are different in Abelmoschus corolla grown in different areas of Jiangsu Province.

关 键 词：黄蜀葵花 金丝桃苷 杨梅素 槲皮素 HPLC法 

分 类 号：R927.2[医药卫生—药学]