结核性胸水中PPD特异性多功能效应型记忆T细胞表型和功能研究  

A study on the phenotypic characterization and function of PPD specific muli-functional effector memory T cells in pleural fluids

作　　者：乔丹[1,2] 李丽[1,2] 李琴[1,2] 劳穗华[3] 张贤兰[3] 吴长有[1,2] 

机构地区：[1]中山大学中山医学院免疫学教研室 [2]中山大学"热带病防治研究"教育部重点实验室,广州510080 [3]广州市胸科医院,广州510095

出　　处：《中国免疫学杂志》2010年第12期1114-1118,共5页Chinese Journal of Immunology

基　　金：十一五基金(2008ZX10003011);国家自然科学基金资助项目(30872300)

摘　　要：目的:探讨结核性胸水中是否存在纯化蛋白衍生物(PPD)特异性T细胞,并对这群PPD特异性T细胞的表型特征和功能进行研究。方法:体外用PPD刺激结核性胸水细胞(PFCs),结合表面分子和细胞内因子染色方法,分析结核性胸水细胞中PPD特异性的T细胞的表型特征和功能。结果:在不刺激的条件下,结核性胸水细胞几乎不产生细胞因子。PPD刺激结核性胸水细胞后,主要使CD4+T细胞产生Th1细胞因子。进一步分析Th1细胞因子产生之间相互关系,结果表明PPD特异性CD4+T细胞大多同时分泌两种细胞因子。表型分析表明,结核性胸水细胞中分泌Th1细胞因子的主要是CD45RA-CD62L-CD27-CCR7-CD4+T细胞,即效应型记忆CD4+T细胞。结论:结核性胸水中存在PPD特异性CD4+T细胞,这群细胞是多功能效应型记忆CD4+T细胞,而且可能对结核菌的控制和清除有重要作用。Objective：To explore whether PPD specific T cells exist in pleural fluids from patients with tuberculosis pleurisy and analyze the phenotypic characterization and function of these cells.Methods：Pleural fluid cells were incubated with PPD in vitro.Cell surface staining and intracellular staining were used to analyze the production of cytokines by PPD specific T cells and the phenotypic characterization.Results：Pleural fluid cells didn＇t produce any cytokine in medium alone.Following the stimulation with PPD in vitro,CD4＋ T cells in pleural fluids were the main source of PPD specific Th1 cytokines.Further we investigated the correlations among the Th1 cytokine production,and found that these PPD specific CD4＋ T cells mainly coexpressed two Th1 cytokines（IFN-γ,IL-2 or TNF-α）.Phenotypic analysis indicated that these cytokine-producing pleural fluid cell were mainly polyfunctional effector memory CD4＋ T cells with the phenotype of CD45RA-CD62L-CD27-CCR7-.Conclusion：Our data indicats that PPD specific CD4＋ T cells exist in pleural fluids from patients with tuberculosis pleurisy.These cells are polyfunctional effector memory CD4＋ T cells,which could produce two or more Th1 cytokines.They may play an important role in the control of mycobacterium tuberculosis infections.

关 键 词：PPD 胸水细胞 TH1细胞因子 表型 结核性胸膜炎 

分 类 号：R392[医药卫生—免疫学]