基于一步电沉积层状甘氨酸-N,N-双甲基膦酸锆/海藻酸钠/血红蛋白的生物传感器的研究(英文)  

One-Step Construction of the Biosensor Based on Layered Zirconium Glycine-N,N-dimethylphosphonat-Sodium Alginate-Hemoglobin Composite Film Formed by Electro-co-deposition

作　　者：徐静[1] 刘昌华[1] 滕瑛丽[1] 午海霞[1] 吴宗方[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2010年第6期54-58,共5页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：一种新的有机-无机杂化层状甘氨酸-N,N-双甲基膦酸锆被合成并成功的用于固定血红蛋白.层状甘氨酸-N,N-双甲基膦酸锆/海藻酸钠/血红蛋白成功的电沉积到金电极的表面.血红蛋白的电子转移系(α)和电子转移速率常数(ks)分别为0.91和0.647 s-1,表明了血红蛋白酶和金电极之间的电子转移速率较快.在优化的实验条件下,响应电流与过氧化氢的浓度在1.4μmol/L to 3.5 mmol/L范围内呈良好的线性关系,检出限为4.7×10-7mol/L(S/N=3),传感器具有好的灵敏度、重现性、稳定性.A new organic-inorganic hybrid layered zirconium glycine-N,N-dimethylphosphonate Zr[（O3PCH2）2NCH2COOH]·H2O（ZGDMP） has been synthesized and the intercalation behavior of hemoglobin（Hb） was investigated.In this paper,Hb and biocompatible composite zirconium glycine-N,N-dimethylphosphonate and sodium alginate（ZGDMP-SA） were successfully electro-co-deposited onto the surface of gold electrode.The electrochemical parameters of Hb in the ZGDMP-SA was calculated with the results of the electron transfer coefficient（α） and the apparent heterogeneous electron transfer rate constant（ks） as 0.91 and 0.647 s-1 respectively,indicating good facilitation of the electron transfer between Hb and the modified electrode.The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 1.4 μmol/L to 3.5 mmol/L with a detection limit of 4.7×10-7 mol/L（S/N=3）.

关 键 词：过氧化氢生物传感器 层状甘氨酸-N N-双甲基膦酸锆 海藻酸钠 血红蛋白 

分 类 号：O657.1[理学—分析化学]