SMAO休克兔肠组织气体分子及形态学的动态变化观察  

The Dynamic Observation of NO,CO,H_2S and Morphology in the Intestine on Rabbit During SMAO Shock

作　　者：凌灿[1] 凌丽[2] 张建龙[3] 杜奕冲[4] 何珊[4] 白晶[4] 刘晓洁[4] 

机构地区：[1]新疆医科大学厚博学院机能教研室,乌鲁木齐830011 [2]西南大学信息中心,重庆400716 [3]新疆医科大学基础医学院病理生理学教研室,乌鲁木齐830011 [4]新疆医科大学厚博学院临床2006-4,乌鲁木齐830011

出　　处：《西南师范大学学报（自然科学版）》2010年第6期63-67,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：新疆医科大学大学生创新性实验计划项目(CX2009052)

摘　　要：目的:观察肠系膜上动脉夹闭性休克(SMAO休克)过程兔肠组织一氧化氮(NO)、一氧化碳(CO)、硫化氢(H2S)的动态变化.方法:将40只新西兰大耳白兔随机分为5组:假手术组、缺血60 min组(I组)、缺血60 min再灌注30 min组(I/R30 min组)、缺血60 min再灌注60 min组(I/R60 min组)、缺血60 min再灌注120 min组(I/R120 min组),每组8只.检测肠源性休克过程各组兔动脉血压及肠组织气体分子(NO,CO,H2S)及形态学的动态变化.结果:肠组织NO含量在再灌注组明显增高,且随着再灌注时间的延长增高(p<0.01);CO含量在I组明显增高(p<0.01),再灌注30 min达到最高值,之后随再灌注时间的延长有所降低(p<0.01);H2S含量在I组明显降低(p<0.01),而再灌注各组明显增高(p<0.01),再灌注60 min达到最高值,而再灌注120 min有所降低(p<0.01).肠组织病理学结果显示,肠缺血组可见大量炎性渗出物,脱落的上皮细胞,上皮细胞肿胀,黏膜下血管充血、扩张明显;肠缺血再灌注组损伤明显加重,可见多处出血与坏死.结论:肠源性休克致肠屏障损伤,内毒素移位,随着再灌注时间的延长引起肠组织NO,CO和H2S的动态变化,造成机体损伤加重.Objective： To observe the dynamic changes of NO,CO,H2S in the intestine on rabbit during SMAO shock.Methods： 40 New Zealand big-ear white rabbits were divided into 5 groups randomly： sham operated,ischemic 60 min,I 60 min/R 30 min,I 60 min/R 60 min,I 60 min/R 120 min,8 rabbits in each group.To detect the dynamic changes of NO,CO,H2S in the intestine on rabbit during SMAO shock.Results：The end toxin content of blood plasma in each experimental groups is increased obviously,and it will increase more with the reperfusion time being prolonged（p〈0.01）;the NO content of the intestine is increased obviously in the ischemic group（p〈0.01）;the CO content of intestine in I group is increased obviously,arrived at the topmost in the reperfusion 30 min,with the cutting down in the reperfusion（p〈0.01）;the H2S content of the intestine is decreased obviously in the ischemic group（p〈0.01）,arrived at the topmost in the reperfusion 60 min,but is cut down in the reperfusion 120 min（p〈0.01）.Conclusions： SMAO shock induces end toxemia,the end toxin content was increased endlessly with the reperfusion time being prolonged,then inducing the dynamic change of NO,CO and H2S in the organism,and making the injury aggravated.

关 键 词：SMAO休克 气体分子 动态变化 

分 类 号：R322.35[医药卫生—人体解剖和组织胚胎学]