The Superiority of Like-rocket Immunoelectrophoresis Using in Single Cell Gel Electrophoresis Assay  

仿火箭电泳应用于单细胞凝胶电泳技术的优势(摘要)(英文)

作　　者：穆淑梅[1] 康现江[1] 郭明申[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《Agricultural Science & Technology》2010年第7期17-19,共3页农业科学与技术（英文版）

基　　金：Supported by Natural Science Foundation of Hebei Province(C2008000591)~~

摘　　要：[Objective] The like-rocket immunoelectrophoresis was used to explore a new feasible electrophoresis method for single cell gel electrophoresis assay （comet assay）.[Method] The like-rocket immunoelectrophoresis was used for single cell gel electrophoresis assay to detect DNA damage at single cell level,then it was compared with traditional electrophoresis method to analyze its advantage and disadvantages.[Result] Under cell DNA undamaged state,the results of two electrophoresis methods were consistent.When cell DNA was damaged,the comet tail divergence of some cells under traditional electrophoresis method were drifted,however,the comet tail image of like-rocket immunoelectrophoresis was concentrated and not shifted.[Conclusion] The like-rocket immunoelectrophoresis had some advantages.[目的]研究仿火箭电泳应用于单细胞凝胶电泳技术,为单细胞凝胶电泳技术寻求另一种可行的电泳方法提供参考。[方法]将仿火箭电泳方法应用于单细胞凝胶电泳技术,检验单细胞水平上的DNA损伤,并与传统的电泳方法比较,分析其优劣势。[结果]在细胞DNA未受损状态下,2种电泳方法所得的结果一致;而在细胞DNA受到损伤时,传统的电泳方法下一些细胞的慧尾发散出现漂移,仿火箭电泳方法得到的慧尾图像集中且未发生偏移。[结论]仿火箭电泳方法较传统的电泳方法存在一定优势。

关 键 词：Single cell gel electrophoresis DNA damage Like-rocket immunoelectrophoresis 

分 类 号：Q503[生物学—生物化学]