hBMP2和hVEGF165双基因修饰组织工程骨的构建及检测  

Construction and detection of dual-gene-modified tissue engineering bone with the hBMP2 and hVEGF165

作　　者：范伟[1] 张弓[1] 安洪[1] 蒋电明[1] 倪卫东[1] 

机构地区：[1]重庆医科大学附属第一医院骨科,400016

出　　处：《重庆医学》2010年第24期3317-3319,F0002,F0003,共5页Chongqing medicine

摘　　要：目的构建hBMP2和hVEGF165双基因修饰的组织工程骨并检测其表达情况。方法构建hVEGF165和hBMP2真核表达质粒,并转染至兔成骨细胞,以DPB为支架,构建双基因修饰的组织工程骨,同时采用RT-PCR和Western blot对两种基因表达情况进行检测。结果转染hBMP2和hVEGF165的成骨细胞可以在DPB上贴附生长,并且体外表达时间可持续7 d。结论成功构建双基因修饰组织工程骨,且能有效表达,为其用于大段骨缺损修复奠定了实验基础。Objective Construction of dual-gene-modified tissue engineering bone with hBMP2 and hVEGF165,and detection the genes expression.Methods This experiment is to construct pEGFP/hBMP2 and pEGFP/hVEGF165,which were both transfected into osteoblasts and then build a dual-gene-modified tissue engineering bone in vitro with the DPB as the frame.We detected the genes expression with RT-PCR and Western blot.Results Osteoblasts transfected plasmids of pEGFP/hBMP2 and pEGFP/hVEGF165 could be growthed in DPB,and the genes could continue to express about 7 d.Conclusion The dual-gene-modified tissue engineering bone were constructed successfully,the genes could be effective expressed in this tissue engineering bone,thus laying an experimental foundation for further observation of its revascularization and bone formation in vivo and for the repair of large bone defect.

关 键 词：组织工程骨 血管内皮细胞生长因子 骨形态发生蛋白 基因表达 

分 类 号：R687.34[医药卫生—骨科学] Q786[医药卫生—外科学]