建立实验室内不同凝血检测系统生物参考区间的探讨  

Study on the establishment of biology reference interval of different coagulation detection system in the clinical medicine laboratory

作　　者：徐华建[1] 田小浪[1] 刘劲松[1] 白垚[1] 张莉萍[1] 

机构地区：[1]重庆医科大学附属第一医院检验科,400016

出　　处：《重庆医学》2010年第24期3338-3339,共2页Chongqing medicine

摘　　要：目的以PT、aPTT、Fbg、TT4个凝血项目为例,探讨建立实验室内不同凝血检测系统生物参考区间的方法。方法以美国临床实验室标准化委员会(NCCLS)C28-A2推荐的方法,通过检测120例健康个体,建立适合实验室内不同凝血检测系统的凝血项目生物参考区间,用计算符合率的方法对自建生物参考区间进行评价。结果 CA7000凝血检测系统参考区间为PT10.5～13.7 s、aPTT15～34 s、Fbg 1.5～3.5 g/L、TT15～20 s;STA-R凝血检测系统参考区间为PT 11.0～14.5 s、aPTT 28～44 s、Fbg 1.8～3.8 g/L、TT15.4～19 s。检测系统间临床符合率大于80%。结论采用本方法建立实验室内不同凝血检测系统的生物参考区间科学、可靠,值得推广应用。Objective To explore a suitable method for the clinical laboratory to establish the biological reference intervals of different coagulation detecting systems.Methods According to the recommendation of NCCLS C28-A2,blood coagulograms of 120 healthy individuals were tested respectively by CA7000 and STA-R including PT,aPTT,Fbg and TT.Coincidence was calculated to evaluate the two biological reference intervals.Results The biological reference intervals of CA7000：PT 10.5-13.7 s,aPTT 15-34 s,Fbg 1.5-3.5 g/l,TT 15-20 s.The biological reference intervals of STA-R：PT 11.0-14.5 s,aPTT 28-44 s,Fbg 1.8-3.8 g/L,TT 15.4-19s.The clinical coincidence of CA7000 and STA-R was above 80%.Conclusion The established method of biological reference interval for different coagulation detection systems in our study is scientific,reliable and worthy to be widely applied.

关 键 词：生物参考区间 检测系统 建立 

分 类 号：R446.11[医药卫生—诊断学]