i2000化学发光仪检测HBsAg适用稀释液及最适稀释倍数的选择  

The choice of HBsAg applicable diluent and optimization dilution multiple with i2000 Chemoluminescence Analyzer

作　　者：夏吉荣[1] 邹麟[1] 周春燕[1] 向渝[1] 张莉萍[1] 

机构地区：[1]重庆医科大学附属第一医院检验科,400016

出　　处：《重庆医学》2010年第24期3340-3341,共2页Chongqing medicine

摘　　要：目的探讨i2000化学发光仪检测HBsAg的适用稀释液及最适稀释倍数,为乙型肝炎感染患者的临床诊断和治疗提供准确的检测结果。方法 (1)稀释倍数的选择:按厂家建议的方法将稀释后的1 192例检测结果进行不同稀释倍数的频数统计分析,以确定最适稀释倍数。(2)适用稀释液选择:使用仪器厂家的稀释液、全阴性混合血清、生理盐水、10%小牛血清分别对HBsAg>250 IU/mL的30例不同水平的患者血清进行50、100、500倍稀释后进行检测;将全阴性混合血清、生理盐水、10%小牛血清稀释液的检测结果与厂家提供的稀释液的检测结果作相关性统计分析,选出合适的稀释液及稀释倍数。结果 (1)已稀释检测的1 192例标本中,95%的检测结果在50倍稀释范围内;98%的检测结果在100倍稀释范围内;99%的检测结果在500倍稀释范围内。(2)全阴性混合血清、生理盐水、10%小牛血清稀释的检测结果与厂家稀释液检测结果比较差异均无统计学意义(P>0.05),见表1。结论当HBsAg>250 IU/mL时应首选100倍稀释后检测;如100倍稀释后HBsAg检测结果仍大于250 IU/mL时再作500倍稀释后检测;稀释液除了用厂家稀释液外,全阴性混合血清、生理盐水、10%小牛血清都可作为适用稀释液。Objective To discuss the HBsAg applicable diluent and optimization dilution multiple with i2000 Chemoluminescence Analyzer and provide evidence for diagnosis and therapy of HBV infection.Methods（1） The choice of dilution multiple： The optimization dilution multiple was determined by analyzing statistically frequency of different dilution multiple from 1 192 results which were detected with dilution method suggested by manufacturer in our Clinical Laboratory.（2） The choice of applicable diluent： 30 distinct patient serum（HBsAg〉250 IU/mL）were detected after 50,100,500 fold dilution with manufacturer diluent,mixture negative serum,normal sodium and 10% calf serum.The suitable diluent and dilution multiple were determined by analyzing statistically test results of manufacturer diluent,mixture negative serum,normal sodium and 10% calf serum.Results（1） In 1 192 diluted samples,95% of the test results were within 50 fold dilution range;98% of the test results were in 100 fold dilution range;99% of the test results were in the range of 500 fold dilution range.（2）There were no significantly different between the detection results of manufacturer diluent and those of mixture negative serum,normal sodium and 10% calf serum.（P〉0.05）Conclusion When HBsAg〉250 IU/mL,100 fold dilution is selected firstly.As HBsAg250 IU/mL after 100 fold dilution,500 fold dilution is selected and detected.The manufacturer diluent,mixture negative serum,normal sodium and 10% calf serum are as applicable diluent.

关 键 词：雅培i2000发光仪 HBSAG 稀释液 稀释倍数 

分 类 号：R446.61[医药卫生—诊断学] R512.62[医药卫生—临床医学]