临床微生物检验人员显微镜检查一致性的实施与评估  

Implementation and evaluation of consistency for microscopic examination in clinical microbiology

作　　者：夏云[1] 曹何[1] 严立[1] 张晓恒[1] 张莉萍[1] 

机构地区：[1]重庆医科大学附属第一医院检验科,400016

出　　处：《重庆医学》2010年第24期3346-3347,共2页Chongqing medicine

摘　　要：目的评价临床微生物室内和室间革兰染色和抗酸染色显微镜检查的一致性。方法对同一标本进行革兰染色显微镜检查以及采用CAP质控片进行显微镜检查,以评价同一实验室人员之间的一致性;对通过ISO15189质量认证的3个实验室送检的革兰染色和抗酸染色比对片进行检查,将结果与送检单位比较以考察不同实验室之间显微显微镜检查查的一致性。结果同一实验室人员显微镜检查一致性为96.7%,与其他3个实验室革兰染色显微镜检查一致性分别为90%、85%、87%;抗酸染色显微镜检查一致性分别为90%、80%、100%。结论在严格实行室内质量控制程序和人员培训的实验室,误差因素得到有效的控制,显微镜检查具有很好的一致性,实验室间的结果具有良好的可比性。Objective To evaluate the consistency of Gram stain and Acid-fast stain microscopic examination in different stuff and different microbiological laboratories.Methods The same sample and the CAP control smears were Gram stained and microscopic examined by our work staff.The results were used to evaluate the consistency of different person in our laboratory.The stained smears collected from three laboratories which authorized by ISO15189 were microscopic examinated and the consistency was evaluated by comparing the results of our and sample offering laboratory.Results In total six people were involved in evaluation,the consistencies of Gram stain microscopic examination was 96.7%.The consistency of Gram stain and Acid-fast stain microscopic examination copared with the first,the second and the third laboratory wree 90%,85%,87% and 90%,80%,100% respectly.Conclusion In the laboratory that executing stringent quality insurance and staff training programe,error factors could be effective controlled and then the satisfacted consistency in different staff or different laboratories could be obtained.

关 键 词：显微镜检查 人员比对 

分 类 号：R446.5[医药卫生—诊断学]