水稻内生粘着剑菌的分子系统发育分析  

Phylogenetic Analysis of Endophytic Ensifer adhaerens Isolated from Rice Roots

作　　者：张晓霞[1] 邱服斌[2] 孙磊[3] 马晓彤[1] 姜瑞波[1] 宋未[4] 

机构地区：[1]中国农业科学院资源与区划研究所农业部作物营养与施肥重点实验室,北京100081 [2]山西医科大学公共卫生学院,太原030001 [3]河北大学生命科学学院河北省微生物多样性研究与应用实验室,保定071002 [4]首都师范大学生命科学学院,北京100048

出　　处：《应用与环境生物学报》2010年第6期779-783,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(Nos.30900001,30770069);中国农业科学院农业资源与农业区划研究所中央级公益性科研院所基本科研业务费专项资金(No.901-10);公益性行业(农业)科研专项经费(No.200803029-01);北京市自然科学基金项目(No.5092004)资助~~

摘　　要：通过16S rRNA基因和看家基因recA、atpD、rpoB的序列测定与分析,及(G+C)mol%测定等技术,对分离自河北省滦南县水稻根内的两株细菌进行分子系统发育学研究.根据系统进化树构建及其同源性分析,可确定所分离的两株水稻内生细菌属于剑菌属(Ensifer)系统发育分支;供试菌株的各保守序列与粘着剑菌(E.adhaerens)模式菌种同源性最高,除recA为97.2%外,其他均为99%～100%,可以确定供试菌属于E.adhaerens;对所分离的菌株与大豆、菜豆、三叶草、苜蓿等4种豆科植物进行结瘤试验,结果显示其中一株J3-N55可与苜蓿结瘤.野生型的E.adhaerens菌株与豆科植物结瘤的报道尚为鲜见;本文首次报道从水稻植株内分离到E.adhaerens菌株.Two Ensifer（Sinorhizobium）-like strains isolated from rice（Oryza sativa L.） roots in Luannan County,Hebei,China were investigated with a polyphasic strategy.The 16S rRNA,recA,atpD and rpoB genes were PCR amplified and sequenced,and followed by Neighbour-Joining phylogenetic analysis.The G＋C content was determined by thermal denaturation method.According to the study of phylogenetics,the two strains were clustered in the genus Ensifer（Sinorhizobium）,with the highest similarity to E.adhaerens ranging from 99% to 100%,except for recA gene（97.2%）.To determine the nodulation ability,the nodulation tests were carried out using four different legumes： soybean（Glycine max）,kidney bean（Phaseolus vulgaris）,clover（Trifolium repens）,and lucern（Medicago sativa）.It was found that strain J3-N55 could form nodules successfully on M.sativa.To our knowledge,this is the first report about the endophytic E.adhaerens isolated from rice plant.

关 键 词：水稻内生菌 粘着剑菌 系统发育分析 结瘤试验 

分 类 号：S182[农业科学—农业基础科学]