黄连木居群遗传多样性的SSR标记分析  

SSR Analysis on Genetic Diversity of Natural Populations of Pistacia chinensis Bunge

作　　者：吴志庄[1,2] 张志翔[1] 汪泽军 李金霞[4] 王学勇[4] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]国家林业局竹子研究开发中心,杭州310012 [3]河南省林业技术推广站,郑州450002 [4]河北省林业科学研究院,石家庄050061

出　　处：《应用与环境生物学报》2010年第6期803-806,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家"十一五"科技支撑计划项目(No.2006BA007A04);国家林业局"十一五"科技支撑项目(No.2006BAD18B0101)资助~~

摘　　要：为系统揭示黄连木天然居群在遗传多样性水平上的差异及遗传分化状况,采用SSR(Simple sequence repeats)分子标记对其8个居群进行遗传多样性分析.在建立良好的反应体系基础上,从已有的阿月浑子SSR引物中筛选出9对适合进行黄连木遗传分析的引物,在8个黄连木居群中共检测到43条等位基因,位点平均等位基因数为4.78,平均多态位点百分率达90.28%.各居群内平均有效等位基因数为2.08,平均期望杂合度(He)为0.472,说明各居群的遗传多样性处于中等水平.按检测到的有效等位基因数(Ne)和期望杂合度(He),各居群的遗传多样性由高至低依次为安康>唐县>顺平>辉县>略阳>栾川>林州>涉县.居群间的遗传分化系数(FST)平均为0.319,说明居群内变异是黄连木变异的主要来源,并根据遗传距离将8个居群分为三大类.Simple sequence repeat（SSR） molecular marker systems were used to analyze the genetic diversity and genetic structure of 8 natural populations of Pistacia chinensis.Based on establishment of optimized PCR reaction system,nine pairs of SSR primers were selected from the primers which were designed for Pistacia vera L.Totally,43 alleles at average of 4.78 for per locus were observed in the 8 populations and the average of polymorphic locus percentage（P） was 90.28%.The average of effective allele number per locus was 2.08,and the average of expected heterozygosity was 0.472,which showed the total genetic diversity of the populations at a moderate level.According to effective allele number and expected heterozygosity,the genetic diversity of the 8 populations from high to low were AK〉TX〉SP〉HX〉LY〉LC LZSX.The proportion of genetic differentiation among the populations accounted for 0.319,which indicated that P.Chinensis＇s variation mainly resulted from variation among populations.The 8 populations were divided into three types according to genetic distance.

关 键 词：黄连木 居群 SSR 遗传多性样 遗传分化 

分 类 号：S567.52[农业科学—中草药栽培]